浙江湖州桑叶HPLC指纹图谱的研究  

Development of HPLC-fingerprint analysis for the quality assessment of Folium Mori from Huzhou,Zhejiang

作　　者：吴好好[1] 李凡[1] 钱文春 楼丽静 柳丽萍 曾苏[1] 蒋惠娣[1] 

机构地区：[1]浙江大学药学院药物分析研究室,浙江杭州310058 [2]浙江省湖州市蚕业技术推广站,浙江湖州313000

出　　处：《药物分析杂志》2008年第3期390-393,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：浙江省教育厅(G20050668);浙江省医药局(2006C091);浙江省湖州市科技局资助项目(H20042379)

摘　　要：目的:建立桑叶药材的高效液相指纹图谱,为桑叶药材的鉴别及品质控制提供依据。方法:采用 RP—HPLC 法对浙江湖州不同种植基地、不同采摘时间和不同品种的湖州桑叶进行指纹图谱研究,建立 HPLC 指纹图谱共有模式,并进行聚类分析和相似度比较。结果:HPLC 指纹图谱对桑叶药材中主要成分均得到较好分离,根据聚类分析和相似度分析处理结果,浙江湖州不同种植基地、不同采摘时间和不同品种的桑叶药材指纹图谱相似度较好,但少部分品种的药材指纹图谱有明显差别,从采摘时间看,冬季桑叶之间的相似度较其他季节更好。结论:HPLC—FP 可以作为桑叶质量评价和药材鉴别的依据。Objective：To develop the fingerprint determination method for Folium Mori by HPLC method and to provide basis for identification of Folium Mori. Methods： An RP - HPLC method was used to determine the finger- prints of Folium Mori from different plant sites,harvested time and varieties in Huzhou,Zhejiang. Mutual mode was developed, cluster analysis and similarity calculation were taken for comparison of the samples. Results： Samples from different plant sites,collection time and varieties were of high similarity,though a few samples showed evident difference in fingerprint graphics. Samples harvested in winter had even higher similarity than that of other seasons. Conclusion： Fingerprint of Folium Mori can be an identifying method for Folium Mori.

关 键 词：桑叶 指纹图谱 HPLC 

分 类 号：R917[医药卫生—药物分析学]