高效液相色谱分离柱后化学发光法同时测定菟丝子中黄酮类成分  

Simultaneous determination of flavonoids in Semen Cuscutae by HPLC with chemiluminescence

作　　者：高智席[1] 周光明[2] 唐国风[1] 刘焱[1] 

机构地区：[1]遵义师范学院化学系,贵州遵义563002 [2]西南大学化学化工学院,重庆400715

出　　处：《药物分析杂志》2008年第3期476-479,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：贵州省教育厅自然科学青年项目(黔教科2007072)

摘　　要：目的:建立高效液相色谱分离柱后化学发光法测定菟丝子中芦丁、山柰素、槲皮素和异鼠李素含量。方法:基于在氢氧化钠碱性介质中铁氰化钾可以直接氧化芦丁、山柰素、槲皮素和异鼠李素产生化学发光。色谱柱:Hypersil ODS(5μm,4.6 mm×150 mm),流动相:乙醇-乙腈-水-磷酸(21:22:55:2),流速:1mL·min^(-1),柱温:25℃。结果:芦丁、山柰素、槲皮素和异鼠李素浓度分别在0.2～5μg·mL^(-1)(r=0.9990),0.1～15μg·mL^(-1)(r=0.9991),0.1～100μg·mL^(-1)(r=0.9994),0.3～200μg·mL^(-1)(r=0.9998)范围内,与峰面积有良好的线性关系;检测限(S/N=3)分别为0.02,0.08,0.08,0.03μg·mL^(-1)。结论:水法简便、快速、有效,灵敏度高,首次用于菟丝子中黄酮类成分的测定,取得了很好的结果。Objective ： To establish an HPLC with post - column chemiluminescence method for the determination of rutin, quereetin, kaempferol and isorhamnetin in Semen Cuseutae. Methods：The procedure was based on the ehemiluminescent enhancement by rutin, quereetin and kaempferol and isorhamnetin of the K3Fe（CN） 6 system in an alkaline medium. An Hypersil ODS column （5μm,4.6mm×150mm）was used, and the mobile phase consis-ted of carbinol - methyl cyanide - water - phosphoric acid （21 ： 22： 55：2 ）. Flow rate was 1mL·min^-1, the column temperature was controlled at 25℃. Results ： The standard curves of rutin, quercetin , kaempferol and isorh-amnetinwere linear in tile concentration ranges 0.2 -5μg·mL^-1（r =0.9990）,0.1 - 15μg·mL^-1 （r =0.9991）,0.1 -100μg·mL^-1（r=0,9994）and 0.3-200μg·mL^-1（r=0.9998）.The detection limits was 0.02μg·mL^-1,0.08μg·mL^-1,0.08μg·mL^-1 and 0.03μg·mL^-1（S/N：3）.Conclusions：Themethod has been successfully applied to the determination of trace rudimental flavonoids in Semen Cuseutae, which has been proved to be accurate, high sensitivity, simple effective and feasible.

关 键 词：高效液相色谱 化学发此 菟丝子 数酮类成分 

分 类 号：R917[医药卫生—药物分析学]