嗜线虫杆菌接菌量对小卷蛾线虫产量的影响  

EFFECT OF XENORHABDUS NEMATOPHILUS STRAIN A24 ON THE YIELD OF STEINERNEMA CARPOCAPSAE

作　　者：杨秀芬[1] 杨怀文[1] 

机构地区：[1]中国农业科学院生物防治研究所

出　　处：《中国生物防治》1997年第4期163-165,共3页Chinese Journal of Biological Control

摘　　要：本文报道了嗜线虫杆菌Ａ２４品系不同培养时间和接菌量对小卷蛾线虫产量的影响。结果表明：共生菌不同培养时间１６、２０、２４、２８和３２小时对线虫产量没有影响；在其它培养条件相同的情况下，接菌量大，线虫产量高，但每８０ｇ线虫培养基接菌量达到３．３６×１０９时，线虫产量不再随着接菌量的增加而增加，而是稳定在一定的水平。本实验的结果表明：每８０ｇ线虫培养基对共生菌需求量的临界值为３．３６×１０９个细胞。This paper reported the effect of various cultural periods and inoculum size of Xenorhabdus nematophilus on the juvenile yield of Steinernema carpocapsae A24. The symbiotic bacterial cultural periods did not affect the yield of S.carpocapsae cultured for 16, 20, 24, 30 and 32h. The inoculum sizes, however, affect the juvenile yield of S.carpocapsae significally. The nematode yield increased with the increasing of the inoculum size. The optimum inoculum size of X.nematophilus A24 was 10 9 cells for 80g medium with 2.96×10 7juveniles. When the inoculum size was made up to 10 10 cells, the juvenile yield remained at the same level as that of 10 9 cells.

关 键 词：共生菌 昆虫病原线虫 培养 

分 类 号：S476.15[农业科学—农业昆虫与害虫防治]