K_(88).K_(99)工程菌不同佐剂苗对鸡免疫效果的比较  

Comparison of Immune Effect on Chickens by Using Different Adjuvant Vaccine of K 88 K 99 Engineering Strain

作　　者：李学伍[1] 杨艳艳[1] 郭成留[1] 闫玉河[1] 邓瑞广[1] 张改平[1] 郭军庆[1] 李青梅[1] 陈直[1] 袁慧君 

机构地区：[1]河南省农业科学院畜牧兽医研究所

出　　处：《河南农业科学》1997年第11期35-37,共3页Journal of Henan Agricultural Sciences

基　　金：河南省科技攻关项目

摘　　要：采用白油佐剂、弗氏不完全佐剂、氢氧化铝佐剂，分别同抗原（Ｋ８８Ｋ９９工程菌）混合、乳化，制成不同的佐剂苗。以ＰＢＳ（ｐＨ７．２、０．０１ｍｏｌ／ｌ）悬浮的细菌培养物为对照，分别对产蛋鸡进行免疫，用琼扩及ＥＬＩＳＡ检测卵黄抗体。结果表明，白油苗能够诱导产生高效价（１∶１２８）卵黄抗体，而且稳定时间在３０天内。The laying chickens were immunized with different adjuvant vaccines made from the mixtures which contained bacteria cultures(antigens)and White oil adjuvant,Freund's adjuvant incomplete,Al(OH) 3 adjuvant respectively,with the bacteria cultures resuspended in an appropriate volume of PBS(pH 7.2、0.01 mol/l)as the control. The egg antibodies were detected with Immuno-double diffusion and ELISA.The results indicated that the White oil adjuvant vaccine could induce the highest level egg antibodies and the highest level of egg antibodies was stable within 30 days.

关 键 词：佐剂 抗体 工程菌 鸡 免疫效果 

分 类 号：S852.4[农业科学—基础兽医学] S859.797[农业科学—兽医学]