甘肃黄芪毛蕊异黄酮对血管内皮细胞ACE，ACE2表达的影响  

Regulation Effect of Calycosin Extracted from Astragalus membranaceus of Gansu Province on the Expressions of ACE/ACE2 in Vascular Endothelial Cells

作　　者：宋瑞霞[1] 余静[1] 杨丽丽[1] 常鹏[1] 郭雪娅[1] 李秀丽[1] 汪汉卿[2] 董晨明[3] 

机构地区：[1]兰州大学第二医院心内科,兰州730030 [2]中国科学院兰州化物所天然药物研发中心,兰州730000 [3]兰州大学第二医院危重病医学科,兰州730030

出　　处：《中国药学杂志》2008年第8期594-597,共4页Chinese Pharmaceutical Journal

基　　金：甘肃省科学事业费项目(QS041-C33-17);甘肃省自然科学基金项目(ZS031-A25-054-E)

摘　　要：目的观察甘肃黄芪毛蕊异黄酮对体外培养的人脐静脉内皮细胞(HUVECs)中血管紧张素转换酶(ACE)和血管紧张素转换酶2(ACE2)蛋白及其mRNA表达的影响。方法体外培养HUVECs,建立内皮细胞损伤模型,分为对照组,血管紧张素Ⅱ(AngⅡ)组(1×10-6mol.L-1),AngⅡ+毛蕊异黄酮不同剂量组(10,1,0.1mg.L-1),采用HE染色观察内皮细胞的形态,免疫组化(SP法)检测ACE,ACE2蛋白的表达,RT-PCR检测ACE,ACE2mRNA的表达。结果①与正常对照组比较,AngⅡ可促进ACE蛋白,mRNA的表达,减弱ACE2表达(P<0.05);②与AngⅡ组相比,毛蕊异黄酮可增强ACE2表达,抑制ACE分泌,并呈剂量依赖性(P<0.05)。结论甘肃黄芪毛蕊异黄酮通过增强内皮细胞ACE2表达,抑制ACE的分泌,减轻AngⅡ所致的体外培养HUVECs的损伤,具有保护内皮细胞的作用。OBJECTIVE To investigate the effects of calycosin on the protein and gene expressions of angiotensin-converting enzyme （ACE） and angiotensin-converting enzyme 2 （ACE2） in human umbilical vein endothelial cells （HUVECs）. METHODS Cultured HUVECs were divided randomly into 5 groups ： control, angiotensin II （ Ang II ） （ 1×10^- 6 mol· L^-1 ）, Ang II （ 1 × 10^-6 mol· L^-1） ＋calycosin （10, 1, 0. 1 mg · L^-1 respectively）. Morphology of vascular endothelial cells was studied by common microscope with hematoxylin and eosin double staining. Changes in both protein and gene expression of ACE, ACE2 were detected with immuno- histochemistry analysis and reverse transcription polymerase chain reaction （RT-PCR） technique respectively. RESULTS ①Compared with controls, Ang II （1×10^-6mol· L^-1 ） promoted both protein and gene expressions of ACE and inhibited the expression of ACE2 in HUVECs （ P 〈 0. 05 ）. ②Calycosin induced a concentration dependent increase of both protein and gene expressions of ACE2 （ P 〈 0. 05 ）, downregulated the expression of ACE （ P 〈 0.05 ）. CONCLUSION Calycosin from Gansu Astragalus membranaceus showed a protective effect on HUVECs damage by inhibiting the product of ACE and increasing the expression of ACE2. The results suggest that calycosin may play an important role in prevention and treatment of vascular disease.

关 键 词：血管紧张素Ⅱ 人脐静脉内皮细胞 毛蕊异黄酮 血管紧张素转换酶 血管紧张素转换酶2 

分 类 号：R965[医药卫生—药理学]