基于激发荧光光谱的浮游植物分类测量方法  

The phytoplankton classified measure based on excitation fluorescence spectra technique

作　　者：王志刚[1,2] 刘文清[2] 张玉钧[2] 司马伟昌[2] 刘建国[2] 

机构地区：[1]中国科学技术大学地球和空间科学学院,安徽合肥230026 [2]中国科学院安徽光学精密机械研究所,中国科学院环境光学与技术重点实验室,安徽合肥230031

出　　处：《中国环境科学》2008年第4期329-333,共5页China Environmental Science

基　　金：国家“863”项目(2007AA061502);安徽省人才培养及科研带头人专项基金

摘　　要：提出了一种基于激发荧光光谱的浮游植物快速分类测量方法．根据激发荧光光谱特征将淡水浮游植物分成蓝色组、绿色组和褐色组3组．选用铜绿微囊藻、小球藻和脆杆藻分别作为蓝色组、绿色组和褐色组的代表，通过对标准纯种培养体和混合培养体的全波长激发荧光光谱进行多元线性回归计算，得到混合培养体各组分的叶绿素a浓度．结果表明，计算得到的铜绿微囊藻、小球藻和脆杆藻浓度的误差分别为1．67％～12．70％，0．91％，12．70％，6．11％-40．20％，平均误差为5．44％、6．44％和20．71％．使用带宽20，10，4nm的4波段（中心波长为440，480，520，610hm）代替全波长激发荧光光谱计算表明，3种藻类组分误差与全波长激发荧光光谱计算结果误差十分相似．20，10hm波段宽度铜绿微囊藻、小球藻和脆杆藻的平均误差分别为5．57％、7．16％、20．52％和7．13％、7．42％、20．02％；4nm波段宽度的平均误差显著增大，分别为8．85％，13．07％，30．67％．A rapid classified measuring method for phytoplankton based on excitation fluorescence spectra was put forward. Based on the excitation fluorescence spectra characteristics, phytoplankton in fresh water was divided into three spectra groupsblue group, green group and brown group. Selecting Microcystis aemginosa, Chlorella vulgaris and Fragilaria sp. as the representative of blue group, green group and brown group, chlorophyll a concentration of each component in mixed phytoplankton cultures had been calculated by multivariate linear regression on the entire wavelength excitation fluorescence spectra of standard pure phytoplankton cultures and mixed phytoplankton cultures. The errors of the concentration of Microcystis aemginosa, Chlorella vulgaris and Fragilaria sp. were 1.67%-12.70%, 0.91%-12.70% and 6.11%-40.20% respectively, average values were 5.44%, 6.44% and 20.71%. Using four wave bands （central wavelength： 440, 480, 520, 610nm） of bandwidth 20, 10. 4nm for multivariate linear regression instead of entire wavelength excitation fluorescence spectra, the calculation showed that the errors of three phytoplankton components were very similar with the entire wavelength excitation fluorescence spectra results. For 20. 10nm bandwidth, average errors of Microcystis aemginosa, Chlorella vulgaris and Fragilaria sp. were 5.57%. 7.16%, 20.52% and 7.13%. 7.42%, 20.02% respectively. Average errors of 4nm bandwidth were obviously large than that of 20, 10nm, which were 8.85%, 13.07% and 30.67% respectively.

关 键 词：浮游植物 激发荧光光谱 活体荧光 环境监测 

分 类 号：X824[环境科学与工程—环境工程]