高效液相色谱-串联质谱法测定中药制剂中西地那非的含量  

Determination of Sildenafil in Traditional Chinese Medicines by HPLC/MS/MS

作　　者：左海根[1] 占春瑞[1] 罗晓洪[1] 

机构地区：[1]江西出入境检验检疫局,南昌330002

出　　处：《中国药学杂志》2008年第7期541-544,共4页Chinese Pharmaceutical Journal

摘　　要：目的建立一种简单、快速、灵敏、准确地鉴定中药制剂中是否添加西地那非的高效液相色谱串联质谱(LC/MS/MS)的分析方法。方法样品经C18(4.6mm×250mm,5μm),流速200μL.min-1,乙腈-0.05mol.L-1乙酸铵(1:1)流动相进行分离。在ESI正离子采集模式下,采用LC/MS/MS的多反应监测(MRM)对西地那非进行定量监测,采用外标法定量。结果本方法满足分析要求,并对12种市场上中药中西地那非进行定量测定。结论目前市场中药制剂中存在添加西地那非的现象。OBJECTIVE A method of HPLC/MS/MS was developed for the identification of sildenafil added in Chinese traditional medicines.METHODS HPLC Separation was carried out on a C18（4.6 mm×250 mm,5 μm） column using an isocratic mobile phase with the flow rate 200 μL·min^-1.Detection is performed on API 3000 LC/MS/MS system with ESI resources in positive ion acquisition mode and qualified in multiple reaction monitoring（MRM） mode.RESULTS This method is simple,rapid,sensitive and reliable and has been successfully applied to identify sildenafil in 12 kinds of related Chinese traditional medicines.CONCLUSION Sildenafil were added in some Chinese traditional medicines.

关 键 词：高效液相色谱串联质谱 西地那非 中药 

分 类 号：R917[医药卫生—药物分析学]