用物种专化DNA重复序列作分子标记检测导入小麦的大赖草染色质  

Detection of Leymus racemosus Chromatins Transf ered to Common Wheat Using Species-specific DNA Repeats as Molecular Markers

作　　者：柴守诚[1] 刘大钧[2] 陈佩度[2] 齐莉莉[2] 李万隆[2] 

机构地区：[1]西北农业大学小麦研究所,陕西杨陵712100 [2]南京农业大学细胞遗传研究所,江苏南京210095

出　　处：《作物学报》1997年第6期641-645,共5页Acta Agronomica Sinica

基　　金：国家"八五"攻关;"863"项目资助

摘　　要：pLrNAU344,pLrNAU426和pLrNAU647是我们新近从大赖草中克隆到的3个物种专化DNA重复序列。它们都大量存在于赖草属,而小麦中却很少。Southern杂交证明,pLrNAU426和pLrNAU647对导入小麦的8条不同的大赖草染色体(其中3条携带有小麦赤霉病抗性基因)和两条染色体臂都有检测效果,据此可用其作为赖草属染色质的分子标记进一步研究和利用。Three species-specific DNA repeats, pLrNAU647, pLrNAU426 and pLr-NAU344 were isolated from Leymus racemosus in our laboratory recently. They all showed strong hybridization signal with the southern blotted DNAs of Leymus species but very weak signal with those of Triticum aestivum. pLrNAU647 and pLrNAU426 were successfully used as molecular markers in identifying 8 different L. racemosus chromosomes, of which three carry wheat scab resistant genes, and two telochromosomes transfered to common wheat.

关 键 词：DNA重复序列 分子标记 大赖草 巨大冰麦草 小麦 

分 类 号：Q942[生物学—植物学] Q523.03