尾加压素Ⅱ基因多态性与糖尿病肾病的相关性研究  

Association between the polymorphism of urotensin Ⅱ gene and the onset of diabetic nephropathy in Chinese subjects

作　　者：张丹丹[1] 王国英[1] 王晓霞[1] 

机构地区：[1]北京大学第三医院内分泌科,100083

出　　处：《实用医学杂志》2008年第7期1091-1095,共5页The Journal of Practical Medicine

摘　　要：目的:探讨尾加压素Ⅱ基因多态性与中国汉族人群糖尿病肾病易感性的相关性。方法:采用多聚酶链反应与限制性内切酶长度多态性方法(PCR-RFLP)检测尾加压素Ⅱ基因C3836T、G605A多态性。选取340例2型糖尿病患者,其中糖尿病肾病患者195例(病例组),糖尿病未合并肾病者145例(对照组)。结果:糖尿病肾病患者和糖尿病未合并肾病的患者相比,T3836T的基因型频率显著升高(P<0.05),3836T等位基因频率在病例组也显著高于对照组(P<0.01)。但病例组与对照组相比,A605A基因型频率和等位基因频率显著降低(P<0.05)。3836T和A605A基因型可能与糖尿病肾病发病相关(OR=2.134,95%CI=1.085～4.198,P=0.028;OR=0.381,95%CI=0.203～0.713,P=0.003),同时目前的结果也表明T3836T和G605G基因型对糖尿病肾病的血脂指标有影响。结论:尾加压素Ⅱ基因中等位基因3836T和605G可能作为独立危险因素在中国汉族人群中糖尿病肾病的发生和发展中起重要作用。Objective The aim of our study was to investigate the possible effect of urotensin Ⅱ gene polymorphism on development of diabetic nephropathy （DN） in Chinese subjects. Methods Polymerase chain reaction and restriction fragment length polymorphism （PCR-RFLP） were used to examine C3836T and G605A polymorphisms in urotensin Ⅱ （UTS2）gene. We then studied these two SNPs in total 340 Chinese subjects with type 2 diabetes mellitus （T2DM）patients. Of these, 195 patients had DN（case patients）, 145 were considered as control subjects （patients without DN）. Results The genotype frequency of T3836T was significantly higher in patients with DN than patients without DN（P 〈 0.05）. And the allele frequency of 3836T was also significantly higher in DN than without DN group （P 〈 0.01 ） But, the genotype and the allele frequencies of A605A was significantly lower in patients with DN than control subjects （P 〈 0.05）. T3836T and G605A genotypes may showed strongly association with diabetic nephropathy. （0R=2.134,95% CI=1.085- 4.198, P=0.028 ; 0R=0.381, 95 % CI=0.203-0.713, P = 0.003）. Maybe the A605A genotype decreased the risk of diabetic nephropathy. Also present results suggested that T3836T and G605G genotypes had an effect on blood lipids given exposure to diabetic nephropathy. Conclusion The present of alleles 3836T and 605G in homozygosity in C3836T and G605A polymorphisms of the UTS2 gene are independent risk factors for the development of DN in Chinese population.

关 键 词：糖尿病肾病 2型糖尿病 尾加压素Ⅱ 基因多态性 

分 类 号：R587.2[医药卫生—内分泌]