干化学法测定血清茶碱的方法学评价  

Evaluation of dry-chemistry method in detecting serum theophyline

作　　者：徐立风[1] 刘涤瑕[1] 董凤珍[1] 唐德羽[1] 

机构地区：[1]安徽省黄山市第532医院检验科,245041

出　　处：《实用医学杂志》2008年第7期1227-1228,共2页The Journal of Practical Medicine

摘　　要：目的:评价干化学法测定血清中茶碱浓度的可行性。方法:应用全自动干化学分析仪对30例服用茶碱的患者血清进行测定,并与免疫荧光偏振法进行比较,同时对干化学测定法进行方法学评价。结果:茶碱浓度在5.0～200!g/mL范围内线性良好(r=0.9985)。日内RSD为3.31%～5.85%,日间RSD为4.51%～7.39%;回收率为95.75%～102.33%。与免疫荧光偏振法进行比较,有良好相关性(r=0.9617,P>0.05)。结论:应用本方法测定血清中茶碱浓度重复性好,准确度高,与免疫荧光偏振法的测定结果差异无显著性,且需血量少,操作简便、快速,适合急诊检测。Objective To evaluate the feasibility of dry-chemistry method for detecting the concentration of theophyline in serum. Methods The serum from 30 patients receiving theophyline therapy was detected using a fully automated dry-chemistry analyzer and the outcomes were compared with those determined by fluorescence polarization immunoassay. Results The linear range of the dry-chemistry method was 5.0 ～200 μg/mL （r=0.998 5） ;within and between-run CV were 3.31% ～5.85% and 4,51% ～ 7.39%,respectively; recovery rate was 95.75% ～ 102.33%. Drychemistry method was correlated with fluorescence polarization immunoassay （r=0.961 7 ） and the results measured by the two methods had no statistic difference （P 〉 0.05）. Conclusion Automatic dry chemistry is a fast, accurate, and stable method for theophyline measurement, which is suitable for urgent detection.

关 键 词：茶碱 干化学 免疫荧光偏振分析 

分 类 号：R96[医药卫生—药理学]