PCR-DGGE法评价连续培养模型中肠道菌群的多样性  

Evalution of the intestinal flora diversity in continuous culture model by PCR-DGGE

作　　者：陈廷涛[1] 曾本华[1] 袁静[1] 唐欢[1] 魏泓[1] 

机构地区：[1]第三军医大学基础部实验动物学教研室

出　　处：《中国微生态学杂志》2008年第2期105-107,共3页Chinese Journal of Microecology

基　　金：国家"973"计划(2007CB513007);"十一五"国家科技与支撑计划(2006BAK02A03-2)

摘　　要：目的建立人体肠道菌群连续培养模型，验证连续培养模型培养肠道菌群的可行性和稳定性。方法建立连续培养系统，把正常人粪便接种于发酵罐中培养，通过活菌计数和PCR—DGGE法分析连续培养系统中菌群的多样性变化。结果连续培养系统中菌群数量由接种前的10^10～10^11CFU／ml降至10^8-10^9CFU／ml并最终达到稳态；PCR—DGGE法分析结果表明，菌群的多样性指数稳定，各条带均匀度趋于一致。结论连续培养模型能较好模拟人体肠道微生态环境，再现了肠道菌群组成的多样性，可作为肠道菌群培养及菌群相关性研究的离体模型。Objective To establish the continuous cuhure system of intestinal flora and validate the feasibility and stability of microflora in this model. Methods A male donor＇s fecal was collected for inoculation;Viable count and PCRDGGE analysis method were applied to detect the microflora quantity and diversity in this model contrasting to the fecal. Resuits In the continuous culture model ,the quantity of the bacterium descents from 10^10- 10^11CFU/ml to 10^8 - 10^9CFU/ml and reached stabilization eventually ; The result of the PCR-DGGE analysis indicated that the diversity of the microflora were stabiled and the bands were hardly changed. Conclusions The diversity of the the microflora in the continuous culture model is similar to the fecal;The continuous culture model has successfully simulated the microecological of the human intestinal tract,indicating the continuous culture model could be used as the in vitro model of the flora culture and mieroflora researches.

关 键 词：活菌计数 PCR—DGGE 连续培养系统 

分 类 号：R938.1[医药卫生—生药学]