Toll样受体在双歧杆菌脂磷壁酸诱导结肠癌细胞凋亡中的作用  

Effect of TLRs on lipoteichoic acid of Bifidobacterium inducing colon cancer cells apoptosis

作　　者：刘明方[1] 张吉[2] 陈淑惠[2] 王跃[1] 

机构地区：[1]重庆医科大学病原生物学教研室,重庆400016 [2]重庆医科大学临床检验诊断学省部共建教育部重点实验室,重庆400016

出　　处：《中国微生态学杂志》2008年第2期116-119,共4页Chinese Journal of Microecology

基　　金：重庆市教委科研项目(040308)

摘　　要：目的探讨双歧杆菌脂磷壁酸(LTA)对Toll样受体(TLRs)表达的影响及其与诱导结肠癌细胞凋亡之间的关系。方法用Annexin V检测在双歧杆菌LTA处理前后结肠癌Lovo细胞凋亡的变化;流式细胞术检测Lovo细胞表面TLRs的表达,并用相应的TLRs封闭抗体作用后,Annexin V检测经双歧杆菌LTA诱导的Lovo细胞凋亡的变化。结果经双歧杆菌LTA处理后,结肠癌Lovo细胞发生了明显的凋亡,并有一定的时间和剂量依赖关系;结肠癌Lovo细胞有TLR受体的基础表达,经双歧杆菌LTA处理后,TLR2和TLR4在Lovo细胞上的表达增加,其中尤以TLR2增加更为明显;用相应的TLRs抗体封闭作用后,双歧杆菌LTA诱导Lovo细胞凋亡的能力下降。结论双歧杆菌LTA能诱导肿瘤细胞凋亡,并且TLRs特别是TLR2在LTA诱导肿瘤细胞凋亡中可能发挥着主要作用,TLR4可能仅起着协同作用。Objective To explore lipoteichoic acid（LTA） of Bifidobacterium to affect on inducing tumor apoptosis through investigating the effect of LTA on apoptosis of colon cancer Lovo cells and the expression of Toll-like receptor （TLR） in Lovo cells. Methods The changes of Lovo cells morphology induced by LTA were observed by microscope, and the apoptosis rate of Lovo cells was detected by Armexin V-FITC/PI double marked assay. Flow cytometer was used to detecting the expressions of TLR2 and TLR4 on Lovo cells before and after treatment with LTA, and after dealling with close antibody of TLR2 and TLR4, the apoptosis rates of Lovo cells treated by LTA were measured by Annexin V assay. Results Lovo cells treated with LTA appeared remarkable apoptosis, and showed time-dependent and dose-dependent invariably. The expressions of TLR2 and TLR4 especially TLR2 increased significantly. While after dealling with close antibody of TLR2 and TLR4. The apoptosis rates of Lovo cells treated by LTA decreased. Conclusions LTA of Bifidobacterium is able to induce Lovo cells apoptosis, which shows time-dependent and dose-dependent invariably. TLRs,especially TLR2 maybe play a predominant role in the process of LTA inducing Lovo cells apoptosis,while TLR4 no more than has an assistant effect.

关 键 词：TOLL样受体 结肠癌 凋亡 双歧杆菌 脂磷壁酸 

分 类 号：R378.992[医药卫生—病原生物学]