Transgenic Rape with hrf2 Gene Encoding Harpin_(Xooc) Resistant to Sclerotinia sclerotinorium  

作　　者：MA Ling-li HUO Rong GAO Xue-wen HE Dan SHAO Min WANG Qi 

机构地区：[1]Key Laboratory of Monitoring and Management of Plant Diseases and Pests, Ministry of Agriculture/Department of Plant Pathology,Nanjing Agricultural University, Nanjing 210095, P.R.China

出　　处：《Agricultural Sciences in China》2008年第4期455-461,共7页中国农业科学（英文版）

摘　　要：The objective of this study was to increase the resistance of rape using the method of transformation. The gene hrf2 derived from Xathomonas oryzae pv. oryzicola encoding harpinxooc protein was constructed into transgenic vector pCAMBIA1301. The cotyledonal petiole segments from rapeseed variety Yangyou 4 were infected by Agrobacterium tumefaciens strain LBA4404/pCAMBIA1301-hrf2. Hygromycin-resistant green shoots were obtained. Successful integration of the foreign gene into the genome of the rapeseed variety Yangyou 4 was confirmed by PCR, RT-PCR, and β-glucuronidase analyses. Disease bioassays of transgenic plants revealed an improved resistance of transgenic plants to Rape sclerotiniose. In brief, the hrf2 gene can be transferred into rape using the method of Agrobacterium-mediated transformation, which increased the resistance to Sclerotinia sclerotinorium in the transgenic plant.The objective of this study was to increase the resistance of rape using the method of transformation. The gene hrf2 derived from Xathomonas oryzae pv. oryzicola encoding harpinxooc protein was constructed into transgenic vector pCAMBIA1301. The cotyledonal petiole segments from rapeseed variety Yangyou 4 were infected by Agrobacterium tumefaciens strain LBA4404/pCAMBIA1301-hrf2. Hygromycin-resistant green shoots were obtained. Successful integration of the foreign gene into the genome of the rapeseed variety Yangyou 4 was confirmed by PCR, RT-PCR, and β-glucuronidase analyses. Disease bioassays of transgenic plants revealed an improved resistance of transgenic plants to Rape sclerotiniose. In brief, the hrf2 gene can be transferred into rape using the method of Agrobacterium-mediated transformation, which increased the resistance to Sclerotinia sclerotinorium in the transgenic plant.

关 键 词：hrf2 gene Agrobacterium tumefaciens-mediated transformation transgenic oilseed rape resistance 

分 类 号：S5[农业科学—作物学]