辣椒轻斑驳病毒检测方法的比较  

Comparison of detecting methods of pepper mild mottle Tobamovirus

作　　者：王亚南[1] 王锡锋[1] 

机构地区：[1]中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京100094

出　　处：《中国植保导刊》2008年第4期5-7,共3页China Plant Protection

基　　金：北京市自然科学基金(6052010)

摘　　要：为寻求一种灵敏、经济、快捷检测辣椒轻斑驳病毒(Pepper mild mottle Tobamovirus,PMMoV)的检测方法,以辣椒轻斑驳病毒侵染的甜椒叶片为材料,采用DIG标记的核酸斑点杂交、RT-PCR和DAS-ELISA3种方法检测不同系列稀释倍数的辣椒叶片总RNA和叶片提取液,测试3种方法的稀释限点。结果表明,RT-PCR灵敏度最高,地高辛斑点杂交灵敏度次之,DAS-ELISA灵敏度最低。在3种方法中地高辛斑点杂交方法与其他两种方法相比灵敏度较高,不需要任何特定仪器,而且适合大量样品的检测,所以此方法有很好的应用前景。Pepper mild motile Tobamovirus in pepper leaves was detected by three methods,＂ namely DAS-ELISA, RTPCR, DIG-labeled cDNA dot-blot hybridization and dilution limit values were tested. Results showed that RT-PCR Was the most sensitive, DIG-labeled cDNA dot-blot hybridization was more sensitive than DAS-ELISA.. DIG-labeled cDNA dot-blot hybridization was suitable to be applied when the number of samples was big, because this method was relatively sensitive and didn＇t need special instruments.

关 键 词：辣椒轻斑驳病毒 DAS-ELISA RT-PCR DIG 标记核酸斑点杂交 

分 类 号：S436.418.12[农业科学—农业昆虫与害虫防治]