充气在几种饵料单细胞藻二级培养中的应用  

Application of Air Bubbling Method on Amplified Incubation of Microalgae

作　　者：李锋[1] 毛玉泽[1] 于守团 杜美荣[1] 叶乃好[1] 方建光[1] 

机构地区：[1]中国水产科学研究院黄海水产研究所,山东青岛266071 [2]青岛红岛蛤原良种发展有限公司,山东青岛266114

出　　处：《中国农业科技导报》2008年第2期94-99,共6页Journal of Agricultural Science and Technology

基　　金：国家973项目(2006CB400608);国家863计划项目(2006AA10Z414,2006AA100304);中国博士后科学基金资助项目(2006039313);山东省博士后择优基金项目(200603061);国家自然科学基金项目(40776090,40706050,40706048,30700619)资助

摘　　要：采用实验与生产结合的方法,研究了充气条件下几种饵料单胞藻的生长特性。结果表明,充气能大幅度提高4种饵料单胞藻的生长速度。在充气条件下,新月菱形藻、金藻3011、3012和扁藻的最大培养密度分别为2.957×107,1.7×107,2.355×107和3.5×106细胞/mL,分别是对照组最大培养密度的3.2倍,6.5倍,4.1倍和5倍;实验组(充气)与对照组(不充气)在细胞数目、特定生长率(SGR)、相对生长率(RGR)和绝对生长率(AGR)等方面均有显著差异。二级充气培养的藻液可以直接扩种到大池进行生产培养,能有效延长生长时间,显著提高生产培养效率。Growth characteristics of 4 microalgae in bubble condition were studied using experiment combined with production method. The results showed that bubble incubation could improve the growth rate of 4 microalgae dramatically. Under bubble condition, the maximum cell density of N. closterium,L galbana Parke 3011 ,L galbana Parke 3012 and P. subcordiformis were 2.957× 10^7, 1.7 × 10^7 ,2. 355 × 10^7 and 3.5× 10^6 cell per milliliter, and which was 3.2, 6.5, 4.1 and 5 times of the control group under the same conditions respectively. The experiment group showed significant difference with the control one in cell number, special growth rate （ SGR）, relative growth rate （RGR） and absolute growth rate （AGR）. The air-charging incubation seed can be directly used for amplified culture. Air-charging incubation can effectively prolong the growth period of the algae cell, and remarkably enhance the production efficiency.

关 键 词：单胞藻 充气培养 生长率 

分 类 号：S963.8[农业科学—水产养殖]