液相色谱-质谱联用法检测药品非法添加醋酸泼尼松和地西泮  

Detection of Prednisone Acetate and Diazepam Mixed Into Drug by The Liquid Chromatography-Mass Spectrometry Method

作　　者：王连水[1] 姜建国[1] 张西如[1] 郭瑞锋[1] 

机构地区：[1]河北省药品检验所,石家庄050011

出　　处：《中国药事》2008年第4期326-328,共3页Chinese Pharmaceutical Affairs

摘　　要：建立检测制剂中非法掺入的醋酸泼尼松和地西泮专属性方法。采用液相色谱-质谱联用法。选用Venusil MP C18柱,以0.01mol·L-1醋酸铵-乙腈(45∶55)为流动相,流速为1.0mL·min-1,紫外波长为240nm。对非法制剂的提取液进行液相色谱-质谱联用分析,检出醋酸泼尼松和地西泮,其回收率分别为98.50%,RSD=0.33%和98.73%,RSD=0.56%(n=6)。该方法选择性强,灵敏度高,可作为分析检测非法制剂中的醋酸泼尼松和地西泮。To develop a specific method for the prednisone acetate and diazepam mixed into drug. The liquid chromatography-mass spectrometry method was used. The Venusil MP C18 column was used, The mobile phase was 0. 01mol· L^-1 ammonium acetate solution--acetonitrile （45 ： 55）. The prednisone acetate and diazepam mixed into drug was identified according to the chromatographic behavior and mass spectral data by comparison with those of reference substances. The prednisone acetate and ibuprofen were found in drug. Those average recovery were 98.50% and 98.74% （n=6）. The method is selective and sensitive and can be used to detect the prednisone acetate and diazepam mixed into drug .

关 键 词：醋酸泼尼松 地西泮 液相色谱-质谱联用法 

分 类 号：R917[医药卫生—药物分析学]