牛微卫星标记PCR扩增方法的研究  

Construction of the multiplex PCR to analysis the cattle population genetic variation

作　　者：李向阳[1] 张嘉保[2] 

机构地区：[1]内蒙古民族大学成人教育学院,内蒙古通辽028042 [2]吉林大学实验动物中心,吉林长春130062

出　　处：《黑龙江畜牧兽医》2008年第4期8-11,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家高技术研究发展计划(863计划)项目(2001AA243051);国家自然科学基金项目(30460103)

摘　　要：试验综合利用微卫星多重PCR、DNA池、复合点样和快速银染技术对5个品种牛的群体遗传变异情况进行了分析,建立了一套快速分析家兔群体遗传变异的方法,大大提高了分析效率,缩短了试验时间。试验的各群体家兔遗传变异结果与实际育种事实相符。To analyze the population genetic variation in 5 breeds or strains of domestic cattle, the technology of multi-PCR, DNA pool, multiple loading and speedy silver staining were used. And a set of speedy method to analyze the cattle population genetic variation was constructed, which greatly improved the analytic efficiency . The genetic analyzing result of this study was conformed to the fact of the SRC breeding history.

关 键 词：牛 微卫星 遗传变异 

分 类 号：S813.1[农业科学—畜牧学]