西瓜枯萎病菌毒素培养滤液对西瓜幼苗可溶性蛋白的影响  

Effects of the Filtration of Fusarium oxysporum Toxin on the Soluble Protein in Watermelon Seedlings

作　　者：冯智富[1] 王建明[1] 郑海霞[1] 徐玉梅[1] 

机构地区：[1]山西农业大学农学院,山西太谷030801

出　　处：《安徽农业科学》2008年第10期4162-4163,共2页Journal of Anhui Agricultural Sciences

基　　金：山西省自然科学基金(2006011080)

摘　　要：[目的]为深入研究西瓜枯萎病的抗性机制提供依据。[方法]用西瓜枯萎病尖孢镰刀菌毒素培养滤液浸根处理西农8号和郑杂5号的幼苗,研究该毒素滤液对西瓜幼苗可溶性蛋白的影响。[结果]50%的西瓜枯萎病尖孢镰刀菌毒素培养滤液处理后,感病品种郑杂5号24 h后叶片开始萎蔫,抗病品种西农8号没有明显变化。接种毒素后,郑杂5号的叶部蛋白图谱产生了一条比较明显的新的蛋白条带,分子量为40 KD,根部与茎部的蛋白图谱没有特别明显的变化;西农8号的根部产生了一条分子量为44 KD的蛋白条带,茎部的蛋白图谱没有明显变化。[结论]抗病品种西农8号更容易产生新的特异性蛋白条带。[Objective] The purpose was to supply basis for deeply studying the resistance mechanism to watermelon, Fusariwn wilt. [Method] The seedlings of Xinong 8 and Zhengza 5 were treated by root dipping with the filtration of Fusariwn axysporwm toxin to study its effects on the soluble protein in watermelon seedlings. [Result] After treating by 50% filtration of F. oxysporum toxin, the leaves of susceptible variety Zbengza 5 started to wither af- ter 24 h and that of resistant variety Xinong 8 had no significant change. After inoculation with toxin, a new and more obvious protein band with molecular weight of 40 KD was generated in the protein pattern of Zhengza 5 leaf and there was no especially obvious change in that of its root and stem; a protein band with molecular weight of 44 KD was generated in the protein pattern of Xinong 8 root and there was no obvious change in that of its stem. [ Conclusion] The resistant variety Xinong 8 was more easily to generate newly specific protein band.

关 键 词：西瓜枯萎病 尖孢镰刀菌毒素 可溶性蛋白 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]