mRNA差异显示法在维吾尔族和哈萨克族2型糖尿病患者白细胞差异基因筛选中的应用  

The application of mRNA differential display in screening differential expressedgenes of peripheral blood leucocyte from Uigur and Kazak patients with type 2 diabetes mellitus

作　　者：邹林樾[1] 张朝霞[2] 张琼[2] 魏琴[1] 卓菲娅.克依木 

机构地区：[1]新疆医科大学第一附属医院实验动物科学研究中心,新疆乌鲁木齐830054 [2]新疆医科大学第一附属医院检验科,新疆乌鲁木齐830054

出　　处：《基础医学与临床》2008年第4期344-348,共5页Basic and Clinical Medicine

摘　　要：目的选择、研究三代内未和其他民族通婚的维吾尔族2型糖尿病患者与维吾尔族正常人,哈萨克族2型糖尿病患者与哈萨克族正常人之间外周血白细胞mRNA差异表达,寻找致糖尿病基因和抑糖尿病基因。方法应用荧光mRNA差异显示技术分析、鉴定外周血白细胞的差异表达片段,测序后进行同源性分析。结果Z5、Z8、Z15三个差异表达明显的cDNA片段在正常人中高表达,而在2型糖尿病患者中低表达或不表达。其中Z5、Z8与CREG1基因同源,Z15基因未知。结论三个在两个民族正常人高表达,而患者缺乏或低表达的基因,可能与抑糖尿病基因有关,可能是2型糖尿病的病因之一。Objective To identify the differentially expressed genes in Uigur and Kazak patients with and without type 2 diabetes mellitus. Methods The differentially expressed eDNA bands were isolated by fluorescent mRNA differential display from peripheral blood leucocyte of the Uigur and Kazak patients with type 2 diabetes mellitus and the normal controls. After being cloned, all eDNA fragments were sequenced, then underwent sequence analysis, homogenous comparison,and Northern blot analysis. Results Z5,Z8 ,Z15 differentially expressed eDNA fragments were found. They were over-expressed in the normal controls and were lower or seareed in the Uigur and Kazak patients with type 2 diabetes mellitus. They were selected for sequencing and hybridization. Z5 ,Z8 showed highly homologous to cellular repressor of E1 A-stimulated genes, Z15 are unknown. Conclusion Three differentially expressed genes may have a potential relation with type 2 diabetes mellitus.

关 键 词：维吾尔族 哈萨克族 2型糖尿病 MRNA差异显示 基因 

分 类 号：R587.1[医药卫生—内分泌]