富亮氨酸胶质瘤失活基因在胶质瘤中的表达  

Expression of leucine-rich gene glioma inactivated 1 in glioma

作　　者：徐新女[1] 王金环[1] 王淑杰[1] 郭承承[1] 刘宏胜[1] 卢轶[1] 张益伟[1] 白雪[1] 

机构地区：[1]天津市第一中心医院神经外科,卫生部危重病急救医学重点实验室,300192

出　　处：《中华神经外科杂志》2008年第4期252-254,共3页Chinese Journal of Neurosurgery

基　　金：天津市自然科学基金资助（043607611）

摘　　要：目的探讨胶质瘤组织富亮氨酸胶质瘤失活基因1（LGI1）mRNA的表达及与细胞增殖活性之间的关系。方法采用逆转录聚合酶链反应（RT-PCR）法对30例脑胶质瘤组织、2例脑膜瘤、2例瘤旁及2例颅脑损伤内减压脑组织标本进行半定量检测，同时采用S-P免疫组化法检测Ki-67标记指数。结果脑胶质瘤组的LGI1 mRNA表达水平低于正常脑组织和脑膜瘤（P〈0．05）。脑胶质瘤中，WHO Ⅳ级的胶质瘤LGI1 mRNA表达低于WHO Ⅱ级和Ⅲ级胶质瘤（P〈0．05），与Ki-67标记指数呈负相关关系（r=-0．621，P〈0．01）。结论LGI1基因的表达与Ki-67标记指数呈负相关，提示LGI1与胶质瘤的发生关系密切。Objective To analyze the expression of the mRNA of LGI1 in human glioma tissues and to explore the relationship between the expression of the mRNA of LGI1 and cell proliferation. Method The tissue specimens of 30 gliomas, 2 para-tumors, 2 meningiomas and 2 normal brains from cerebral injury patients with Intracranial decompression were collected from the Neurosurgery Department of Tianjin First Central Hospital and the Tianjin Huanhu Hospital. Special primers were designed according to related exons of LGI1. Six exons were amplified by reverse transcriptase polymerase chain reaction （RT-PCR） and the expression level of LGI1 mRNA was detected by semi-quantify. Ki-67 LI was detected by immunohistochemical analysis. Results The expression level of LGI1 mRNA in group of gliomas was significantly lower than that in group of normal tissues or meningomas （ P 〈 0.05 ）. The expression level of LGI1 mRNA in the gliomas of grade Ⅳ was lower than that in the glioma of grade Ⅱ / Ⅲ（ P 〈 0. 05 ）. The expresson of LGI1 had a negative correlation with WHO grade and Ki-67 LI （ r = - 0. 621, P 〈 0.01 ）. Conclusion LGI1 gene is a tumor suppressor gene, the expression of LGI1 gene has a close correlation with the progress of glioma.

关 键 词：神经胶质瘤 富亮氨酸胶质瘤失活基因1(LGI1) 逆转录聚合酶链反应 KI-67 

分 类 号：R686[医药卫生—骨科学]