^18F-氟赤式硝基咪唑肿瘤乏氧显像实验研究  

Hypoxic agent of ^18F-flurorerythronitroimidazole in human lung adenocarcinoma cell line

作　　者：赵伟[1] 杨国仁[2] 于金明[1] 宋现让[3] 柳永蕾[3] 赵书强[2] 巩合义[1] 王仁本[1] 

机构地区：[1]山东省肿瘤医院放疗五科,济南250117 [2]山东省肿瘤医院PET-CT中心,济南250117 [3]山东省肿瘤医院中心基础实验室,济南250117

出　　处：《中华核医学杂志》2008年第2期128-129,共2页Chinese Journal of Nuclear Medicine

基　　金：国家自然科学基金（30371381）

摘　　要：目的探讨^18F-氟赤式硝基咪唑（FETNIM）在肿瘤乏氧诊断中的应用价值。方法30只SPCA-1人肺腺癌荷瘤BM-B／c裸鼠采用随机数字表法分为A和B2组（鼠数分别为16和14只）。尾静脉注射37MBq^18F-FETNIM后A组分别于注射后0．5，1，2，3h处死裸鼠，取血液、肺、心、肝、脾、肾、肿瘤等组织测量质量，用1计数仪测定放射性计数，计算每克组织百分注射剂量率（％ID／g）；B组用PO2微电极测量肿瘤组织内乏氧情况。结果^18F-FETNIM在肾中代谢最高，在脂肪和骨骼中代谢较低，肿瘤／正常组织的放射性比值较高，且随时间而增加，2h达最高，肿瘤／血液放射性比值为1．69±0．37，肿瘤／肌肉放射性比值为1．57±0．47。HE染色显示瘤内有大量的乏氧坏死组织。组织内PO2微电极测量示肿瘤内均有乏氧，肿瘤内PO21．1～27．7mmHg（1mmHg=0．133kPa）。结论^18F-FETNIM在荷瘤裸鼠体内具有较低的外周代谢，脂溶性较低，能被肿瘤乏氧组织摄取，可用于肿瘤乏氧诊断。Objective The aim of this study was to investigate the value of ^18F-fluoroerythronitromidazole （FETNIM） in detecting tumor hypoxia in vivo. Methods BALB/c nude mice were xenografted with SPCA-1 lung cancer cells. Tumors were allowed to grow to a diameter about 10 ram. In group A （ n = 16） , ^18F-FETNIM （ 37 MBq） was injected from tail vein. Biodistribution of ^18F-FETNIM was studied at 0. 5, 1, 2, and 3 h after tracer injection. Blood, muscle, heart, liver, kidney, fat, lung, and tumor were moved and weighted. The radioactivity was measured with percentage activity of injection dose per gram of tissue （ % ID/g） by a γ-counting well. In group B （ n = 14）, the intratumoral PO2 measurements were obtained by polar graphic oxygen needle probe. Results The radioactivity was highest in kidney and lowest in fat and bone matrix. The maximum tumor to blood （T/B） and tumor to muscle（T/M） ratios were 1.69±0.37 and 1. 57±0.47 at 2 h after injection. Intratumoral PO2 measurements were ranged from 1.1 to 27.7 mm Hg （ 1 mm Hg = 0. 133 kPa） and were 10.2 to 40.5 mm Hg for normal tissue. Conclusion A relatively high ^18F-FETNIM retention ratio indicated ^18F-FETNIM to be a potential agent for detecting tumor hypoxia in vivo.

关 键 词：硝基咪唑类 同位素标记 氟放射性同位素 肿瘤 细胞低氧 小鼠 裸 

分 类 号：R686[医药卫生—骨科学]