乙型肝炎导向干扰素在荷瘤鼠体内的分布实验  

Study on the Distribution of the Targeting Interferon in Nude Mice Carrying 2, 2, 15 Cells in vivo

作　　者：曹建彪[1] 胡国茂[2] 秦绍明 

机构地区：[1]北京军区总医院消化中心,北京100700 [2]北京生化免疫制剂中心放射免疫室

出　　处：《中国生化药物杂志》1997年第5期229-231,共3页Chinese Journal of Biochemical Pharmaceutics

摘　　要：将2，2，15细胞接种裸鼠，制得抗乙型肝炎病毒（HBV）动物模型。用125I标记乙型肝炎导向干扰素（T－IFN），观察放射标记物（125I－T－IFN）在荷瘤鼠体内的分布情况，以评价T－IFN在体内的抗HBV导向治疗作用。结果显示，125I－T－IFN在各组织浓度顺序依次为：瘤＞心＞肝＞脾＞肾，且瘤组织与其它各组织比较，有明显差异（P＜0．01）。结果表明，在HBV动物模型体内，T－IFN相对浓集于可分泌HBsAg的瘤区。实验结果显示了T-IFN导向治疗HBV感染的可行性。The animal models with HBV can be obtained by the nude mice injected with 2, 2, 15 carci noma cells. In order to evaluate the anti-HBV effects of targeting interferon (T-IFN) in vivo, T-IFN was labeled with 125I and the distribution of 125I-T-IFN in nude mice carrying 2, 2, 15 cells. The re sults showed that the concentration of 125I-T-IFN in tissues of the mice was in an order of carcinoma> heart>liver>spleen>kidney, and 125I-T-IFN in carcinoma was significantly higher than that in the others (P<0. 01). The results indicate that T-IFN can concentrate in the area that can secrete HB sAg.

关 键 词：乙型肝炎 导向干扰素 2 2 15细胞 碘125 T-IFN 

分 类 号：R978.7[医药卫生—药品] R392－33[医药卫生—药学]