白细胞介素6与钙感应性受体基因多态性对青春期女童骨量增长的交互作用  

Effect of interleukin-6 and calcium sensing receptor gene polymorphisms on bone mass accrual in Chinese adolescent girls

作　　者：李星[1] 何国鹏[1] 苏宜香[1] 

机构地区：[1]中山大学公共卫生学院营养系,广州510080

出　　处：《中华医学遗传学杂志》2008年第2期136-140,共5页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金（30571569）

摘　　要：目的探讨白细胞介素6（interleukin-6，IL-6）与钙感应性受体（calcium sensing receptor，CASR）基因多态性对青春期女童骨量增长的交互作用。方法 选择228名9～11岁半未月经初潮的健康女童进行两年追踪，采用双能X线骨密度仪检测对象追踪前后全身、左侧近端股骨（包括股骨颈、大转子、粗隆间和华氏三角区）和L1-L4腰椎骨矿含量（bone mineral conlenl，BMC）和骨密度（bone mineral density，BMD），采用聚合酶链反应-限制性片段长度多态性技术检测IL-6—634C／G位点多态性，等位基因特异性突变分离扩增-PCR技术检测CASR A986S位点多态性。结果 176名女童完成整个研究。IL-6基因-634C／G和CASR基因A986S位点多态性与青春期女童骨量增长有关联，IL-6基因-634C／G位点CG＋GG基因型女童全身和股骨大转子BMD较CC基因型分别低25．7％和20．6％，CASR基因A986S位点AS＋SS基因型女童L1-L4腰椎BMC和华氏三角区BMD增长率较AA基因型分别低14．9％和51．3％，差异有统计学意义（P〈0．05）。交互作用分析发现，同时具有IL-6基因-634C／G位点G等位基因和CASR基因A986S位点S等位基因的女童，其股骨颈和L1-L4腰椎BMC增长率最低。结论同时具有IL-6基因-634C／G位点G等位基因和CASR基因A986S位点S等位基因的女童，可能是低骨量增长的危险人群。Objective To investigate the effect of interleukin-6 （IL-6） and calcium sensing receptor （CASR） gene polymorphisms on bone mass accrual in Chinese adolescent girls. Methods A total of 228 premenarche Chinese girls （9-11.5 years） were recruited for a 2-year study. Bone mineral densities （BMD） and bone mineral contents （BMC） in the total body, total left hip including femoral neck, trochanter, intertrochanter and Ward＇ s triangle area, and lumbar spine （L1-L4） were measured by DEXA. The - 634C/G polymorphism of IL-6 gene was detected by PCR-restriction fragment length polymorphism and A986S polymorphism of CASR gene was detected by allele-special mutagenically separated amplication-PCR. Results There were 176 available subjects when the 2-year study was completed. The - 634C/G polymorphism of lL-6 gene and A986S polymorphism of CASR gene were significantly associated with bone mass accrual after adjusting the potential confounding factors. Girls with CC genotype of IL-6 - 634G/C gene had higher percentage of increase in BMD of total body （P= 0.027） and femoral trochanter （P = 0.028） than those with CG＋ GG genotypes. Girls with AA genotype of CASR gene had greater percentage of increase in L1-L4 lumbar spine BMC （ P = 0.022） and Ward＇s triangle area BMD （ P = 0.049） than their AS ＋ SS counterparts. Subjects with G allele of IL-6 gene in combination with S allele of CASR gene had less increase in BMC of femoral neck and L1-L4 lumbar spine. Conclusion Adolescent girls carrying the G allele of IL-6 gene at - 634C/G and S allele of CASR gene at A986S could potentially be the risk population of lower bone mass accrual.

关 键 词：白细胞介素6 钙感应性受体 遗传多态性 骨量 

分 类 号：R686[医药卫生—骨科学]