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作 者:白松龄[1] 伊凯 刘国成[3] 韩振海[1] 许雪峰 王冬梅 李天忠[1]
机构地区:[1]中国农业大学园艺植物研究所,北京100094 [2]辽宁省果树研究所,辽宁熊岳115009 [3]沈阳农业大学园艺学院,沈阳110161
出 处:《园艺学报》2008年第4期475-480,共6页Acta Horticulturae Sinica
基 金:国家自然科学基金项目(30671443);北京市重点实验室果树逆境生理与分子生物学实验室项目
摘 要:以苹果‘寒富’品种及其亲本为试材,研究其自交亲和性并鉴定其S基因型。田间授粉试验结果显示,‘寒富’品种表现为自花结实,其母本‘东光’品种亦自花结实;而父本‘富士’品种自花授粉结实率极低,初步推断‘寒富’品种的自花结实性可能遗传自其母本‘东光’。利用PCR技术鉴定‘寒富’品种S基因型为S1S9。由于‘东光’品种存在同名异物问题,本研究除日本‘东光’品种外,对辽宁省果树研究所、吉林省果树研究所、沈阳农业大学等单位保存的中国‘东光’品种的S基因型进行了鉴定,发现除辽宁省果树研究所保存的‘东光’品种S基因型为S2S19,与‘寒富’品种无亲缘关系外,其它两个单位保存的‘东光’品种中均存在S9基因型,可能为‘寒富’杂交育种时的原始母本。‘ Hanfu' apple cultivar (Malus x domestica) and its parents were used in this research to study its self-compatibility and identify their S-genotypes. The pollination test in the field showed that the ‘ Hanfu' is self-compatible, and its female parent ' Dongguang' is also self-compatible, but its male parent ' Fuji' is self-sterile as known. The S-genotype of ' Hanfu' was identified as S1 S9 by the PCR-based method. Also, the S-genotypes of all ' Dongguang' which are homonym were identified except the ' Toko' which came from Japanese and was named as ' Dongguang' in Chinese. The results showed the S-genotype of ' Dongguang' from Liaoning Research Institute of Pomology is S2S19, showing no relationship with ' Hanfu' , and the S-genotypes of ' Dongguang' cuhivars from Shenyang Agricultural University, and Jilin Research Institute of Pomology contain S9 allele, suggesting they are probably the female parent of‘ Hanfu'.
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