怀地黄玻璃化和包埋玻璃化法超低温保存  

Cryopreservation of Rehmannia glutinosa (Gaertn.) Libosch by Vitrification and Encapsulation-vitrification

作　　者：李明军[1] 周娜[1] 刘杰[1] 张晓丽[1] 李萍[1] 张楠[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《园艺学报》2008年第4期607-610,共4页Acta Horticulturae Sinica

基　　金：河南省重点科技攻关项目(0623030700)

摘　　要：比较了玻璃化与包埋玻璃化超低温保存怀地黄[Rehmannia glutinosa(Gaertn.)Libosch]‘85-5’带芽茎段的效果。结果表明:4℃低温锻炼5d,在添加二甲基亚砜(DMSO)和乙酰胺的培养基中预培养3d,60%PVS2室温装载25min,PVS20℃脱水30min时,玻璃化和包埋玻璃化两种保存方法均得到本试验条件下最佳结果;两种方法相比,玻璃化法的存活率和再生率均较包埋玻璃化法高,前者的存活率(88.92%)和再生率(64.29%)分别是后者的1.38倍和2.41倍。可见,采用超低温保存怀地黄种质资源是可行的,且玻璃化法优于包埋玻璃化法。The cryopreservation methods of Rehmannia glutinosa （ Gaertn. ） Libosch by vitrification and encapsulation-vitrification were studied. The results showed that： It was optimal cryopreservation condition for the two methods in the experimentation when the materials were trained at 4℃ low temperature for 5 days, then pre-cuhured on the medium with DMSO and acetamide for 3 days, loaded with 60% PVS2 for 25 min at room temperature and dehydrated with PVS2 for 30 min at 0℃. But compared with the materials cyropreserved by the two methods, the survival rate and regeneration rate by vitrification were both higher than those by encapsulation-vitrification. The survival rate （88.92%） and the regeneration rate （64. 29% ） of the former were 1.38 times and 2.41 times higher than the latter, suggesting cryopreservation of Rehmannia glutinosa （Gaertn.） Libosch by vitrification is applicable to the germplasm conservation.

关 键 词：怀地黄 带芽茎段 玻璃化 包埋玻璃化 超低温保存 

分 类 号：S567.234[农业科学—中草药栽培]