高效液相色谱法测定反应香精中的3,4-苯并芘  

Determination of 3,4-benzo[а] pyrene in process flavoring by HPLC

作　　者：武晓剑[1] 李琼[2] 费玠 王丽芳[4] 

机构地区：[1]上海水产大学,上海200090 [2]上海应用技术学院,上海200235 [3]上海香料研究所,上海200232 [4]东华大学,上海201620

出　　处：《食品科技》2008年第4期192-194,共3页Food Science and Technology

基　　金：上海市教委基金资助项目(05D205009)

摘　　要：建立高效液相色谱测定反应香精中3,4-苯并芘的方法,考察前处理方法及色谱条件对实验回收率的影响,确定了C18固相萃取柱为前处理工艺,萃取剂为甲醇,色谱流动相为甲醇∶水=901∶0,流速为1.0 mL/min,DAD检测波长为UV-290 nm。测定反应样品不含3,4-苯并芘,加标实验回收率为76.66%～79.44%,相对平均偏差为1.28%。A method for the HPLC determination of 3,4-benzo[a] pyrene （B＆P） in process flavoring has been proposed, the pre-treatment and the equipment which influenced the experiment were researched and determined, the pre-treatment was C18 SPE column, the extract solvent was methanol, the mobile phase was methanol：water=90：10 with the flow rate at 1.0 mLJmin, the DAD detection wavelength was 290 nm. The samples did not contain 3,4-benzo [a] pyrene, the recovery was 76.66%-79.44%, the RSD of recovery was 1.28%.

关 键 词：高效液相色谱 3 4-苯并芘 反应香精 

分 类 号：TS411[农业科学—烟草工业] O657.72[理学—分析化学]