雌激素对大鼠前列腺平滑肌细胞增殖的影响  

Effect of estrogen on the proliferation of rat prostatic smooth muscle cells

作　　者：罗仪[1] 李世文[1] 沃飞[1] 郑新民[1] 胡礼泉[1] 郑航[1] 胡万里[1] 陈婵[1] 

机构地区：[1]武汉大学中南医院泌尿外科,430071

出　　处：《中华实验外科杂志》2008年第4期500-501,共2页Chinese Journal of Experimental Surgery

基　　金：湖北省自然科学基金资助项目（2007ABA285）

摘　　要：目的观察17β-雌二醇（E2）对大鼠前列腺平滑肌细胞（PSMC）增殖的影响。方法取体重（253±28）g的雄性SD大鼠30只，无菌切取前列腺，应用酶消化法行原代细胞培养。取3—4代传代细胞，分别加入不同浓度E2（0．1—100）nmol／L处理72h，应用流式细胞仪检测细胞周期、细胞凋亡及其相关蛋白Cyclin D1，应用western blot法检测bcl-2和bax表达。结果E2（1、10nmol／L）促进PSMC从G1期向S期过渡，其S期细胞比率分别为（18．50±4．98）％、（21．16±4．83）％，显著高于对照组（12．39±2．64）％（P〈0．05），并伴随Cyclin D1蛋白表达显著增高。而高浓度E2（100nmol／L）则抑制细胞增殖，其S期细胞比率为（7．98±1．92）％，显著低于对照组（P〈0．05），并伴随bax表达显著增加和细胞凋亡率显著升高。结论低浓度E2能够上调CyclinDl表达加速G1期向S过渡从而促进大鼠PSMC增殖，高浓度E2则通过增加bax表达促进细胞凋亡。Objective To investigate the effect of 17β-estradiol （E2） on the proliferation of prostatic smooth muscle cells （PSMC）. Methods The mature male rat PSMC （ subeultured for 3-4 passages） were obtained by enzyme-digesting and exposed to gradient concentrations （0.1-1130 nmol/L） of E2 for 72 h. The progression of cell cycle, the apoptosis and the expression of Cyclin D1 were examined by flow cytometry, bcl-2 and bax proteins were detected by western blot. Results E2 （ 1,10 nmol/L） promoted the PSMC proliferation by accelerating their cell cycle progression from G1 to S phases. The percentage of cells in S phases of the two group were （ 18.50 ± 4.98 ） % and （ 21.16 ± 4.83 ） %, respectively, significandy higher than those in the control group （ 12.39 ± 2.64 ） % （ P 〈 0.05 ） , and the expression of CyclinD1 was siguificandy up-regulated. However, high concentration of E2 （100 nmol/L） inhibited the cell proliferation and percentage of cells in S phase was （7.98 ± 1.92 ） %, significantly lower than in control group ,and the expression of bax and apoptosis rate were significantly increased. Conclusion E2 can promote rat PSMC proliferation by enhancing the expression of CyclinD1 which accelerates G1 to S phase transition and induce apoptosis of the cells by up-regulating the expression of bax.

关 键 词：雌二醇 前列腺 平滑肌细胞 细胞周期 

分 类 号：R285.5[医药卫生—中药学]