血管活性肠多肽基因转导增强阴茎勃起功能  

Vasoactive intestinal polypeptide gene transfer for erectile dysfunction in diabetic rats

作　　者：王华[1] 沈周俊[2] 

机构地区：[1]浙江嘉兴学院医学院附属第一医院泌尿外科,314001 [2]上海交通大学附属瑞金医院泌尿外科

出　　处：《中华实验外科杂志》2008年第4期504-506,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30240034）

摘　　要：目的探讨阴茎海绵体勃起神经递质血管活性肠多肽（VIP）基因转导入糖尿病大鼠阴茎海绵体并表达多量VIP以增强勃起功能的可行性。方法将构建的重组质粒pcDNA3／VIP cDNA注射入链尿佐菌素诱导的糖尿病大鼠阴茎海绵体，在注射3d后测定阴茎海绵体内压（ICP），分别以正常空白、空白、单纯注射缓冲液和单纯注射pcDNA3载体对照。Dot blot法测定阴茎组织中VIP mRNA表达水平。结果糖尿病大鼠阴茎海绵体注射重组质粒后3d，电刺激海绵体神经，ICP较对照组明显升高（P〈0．05）；阴茎组织VIP mRNA表达增多（P〈0．05）。结论成功将重组质粒pcDNA3／VIP cDNA转导入糖尿病阴茎海绵体，VIP mRNA表达增加能增强糖尿病大鼠阴茎海绵体的勃起功能。Objective To determine the feasibility of transfecting penile corpora cavemosa with pcDNA3/VIP cDNA, and whether transfection of VIP cDNA to penis can affect the erectile function in streptozotoein -diabetic Sprague-Dawley rats. Methods The pcDNA3/VIP cDNA was injected into the corpus cavernosum of the diabetic rats. The intracavernous pressure （ICP） was measured at 3rd day after injection. The expression of VIP mRNA in the penile sample was detected by using Dot blot. Results Compared with control animals, the mean amplitude of ICP and expression of VIP mRNA in penis of the VIP-treated rats were increased at 3 rd day after intracavernous injection, respectively （P 〈 0.05 ）. Conclusion VIP cDNA is easily incorporated into corpus cavernosum. The transfer of VIP can increase the ICP of the diabetic rats.

关 键 词：血管活性肠多肽 基因治疗 糖尿病 阴茎 

分 类 号：R285.5[医药卫生—中药学]