根癌农杆菌介导的盐生杜氏藻DsNRT2基因转化紫花苜蓿的初步研究  

Transformation of alfalfa(Medicago sativa L.) by agrobacterium-mediated process with DsNRT2 gene from Dunaliella salina

作　　者：李世林[1] 杨舸[1] 易秀莉[1] 马根[1] 马小珍[1] 李音[1] 曹毅[1] 

机构地区：[1]四川大学生命科学学院四川省生物信息与代谢工程共享实验平台,成都610065

出　　处：《四川大学学报（自然科学版）》2008年第2期409-412,共4页Journal of Sichuan University(Natural Science Edition)

基　　金：四川省杰出青年基金(05ZQ026-029)

摘　　要：利用根癌农杆菌介导法进行紫花苜蓿遗传转化研究.将从抗逆生物盐生杜氏藻中克隆得到的DsNRT2(编码硝酸盐转运蛋白)导入紫花苜蓿"中苜一号"中,在含50 mg/L的卡那霉素的培养基上获得再生苗35株,经PCR和Southern杂交鉴定,得到7株阳性苗,表明DsNRT2基因已整合到苜蓿的基因组中.Alfalfa cultivar ZhongMuYiHao was transformed with DsNRT2 gene from Dunaliella Salina via Agrobacterium-mediated process. 35 regenerated alfalfa plants were obtained on the culture medium with 50 mg/L Kanamyein and 7 putative transgenie plants were obtained by PCR amplification analysis. Southern blot analysis has confirmed that DsNRT2 gene was already integrated into the genome of alfalfa.

关 键 词：紫花苜蓿 DsNRT2基因 转基因植株 

分 类 号：Q78[生物学—分子生物学]