高效阴离子交换-脉冲安培检测色谱法测定人工培养蛹虫草多糖的单糖组成  

Determination of monosaccharide composition of polysaccharides in cultured Cordyceps militaris by high performance anion exchange chromatography with pulsed electrochemical detection

作　　者：杨威[1] 殷茵[1] 宋丽艳[1] 赵昱[2] 于荣敏[1] 

机构地区：[1]暨南大学药学院,广东广州510632 [2]浙江大学药学院,浙江杭州310038

出　　处：《中草药》2008年第4期531-535,共5页Chinese Traditional and Herbal Drugs

基　　金：广州市科技计划项目(2006Z3-E5031)

摘　　要：目的采用高效阴离子交换-脉冲安培检测色谱法测定人工培养蛹虫草多糖的单糖组成。方法以人工培养蛹虫草子实体为原料,利用热水和稀碱液分别对其水溶性多糖成分进行提取,分离得到6种人工蛹虫草水溶性多糖化合物。其中水提部分主要分离得到3种精多糖P50、P70-1和P70-2,碱提部分则分离得到3种精多糖CBP- 1、CBP-2和CBP-3。高效阴离子交换-脉冲安培检测色谱法分别对上述6种精多糖的单糖组成成分进行了测定。结果除CBP-3为葡聚糖外,其他5个多糖均为由D-甘露糖、D-半乳糖和D-葡萄糖组成的杂多糖。结论采用高效阴离子交换-脉冲安培检测色谱法进行多糖化合物的单糖组成成分测定,无需进行衍生化,可达到简便、高效、准确地进行单糖组成分析之目的。Objective To determine the monosaccharide compositions of polysaccharides in cultured Cordyceps militaris by high-performance anion-exchange chromatography with pulsed-electrochemical detection （HPAEC-PED）. Methods Through the application of the hot-water extraction and dilute base extraction methods, taking the cultured C. militaris sporophore as raw matarial, six water-soluble polysaccharides were ohtained from the cultured C. militaris. The polysaccharides named as P50-1, P70-1, and P70-2 were isolated from the hot-water extraction, and CBP-1, CBP-2, and CBP-3 from the base extraction. The six components of the monosaccharide in the polysaccharides were analyzed by HPAEC-PED. Results The results showed these water-soluble polysaccharides all consisted of mannose, glucose, and galactose except CBP-3, which was supposed to be a glucan. Conclusion This method is simple, high efficiency, accurate and can be used to determine the monosaccharide composition of polysaccharides without derivatization.

关 键 词：人工蛹虫草 多糖 单糖 高效阴离子交换-脉冲安培检测色谱 

分 类 号：R284.2[医药卫生—中药学] R286.02[医药卫生—中医学]