ISSR-PCR鉴别绞股蓝属7种植物  

Identification of seven plants of Gynostemma Bl.by ISSR-PCR

作　　者：王翀[1] 周天华[1] 杨雪[1] 郭晶[1] 赵桂仿[1] 

机构地区：[1]西北大学生命科学学院西部资源生物与现代生物技术教育部重点实验室,陕西西安710069

出　　处：《中草药》2008年第4期588-591,共4页Chinese Traditional and Herbal Drugs

摘　　要：目的采用ISSR-PCR技术对绞股蓝属(Gynostemma B1.)绞股蓝G.pentaphyllum、五柱绞股蓝G.pen- tagynum、心籽绞股蓝G.cardiospermum、长梗绞股蓝G.longipes、喙果绞股蓝G.yixingense、疏花绞股蓝G.laxi- florum、广西绞股蓝G.guangxieme 7种药用植物进行DNA分子水平鉴定。方法CTAB法提取绞股蓝属植物总DNA,以57条ISSR引物进行PCR扩增及琼脂糖凝胶电泳分析。结果筛选出产物清晰稳定的14条引物,其中引物UBC-873与UBC-895具有较高的多态性条带比率,均可独立区分7种绞股蓝属植物。结论ISSR-PCR分析可有效区分鉴别常见绞股蓝属植物。Objective To identify seven species of Gynostemma Bl., including G.pentaphyllum, G.pentagynum,G.cardiospermum, G.longipes, G.yixingense,G.laxiflorum, and G.guangxiense, by inter-simple sequence repeat （ISSR） markers. Methods General DNA was isolated from leaves of the seven species in Gynostemma Bl. by CTAB, 57 primers constituted by ISSR were tested for PCR and sepharose electrophoresis. Results Fourteen primers amplified polymorphic bands, the amplification patterns of primers UBC-873 and UBC-895 were higher in terms of polymorphic and amplified band ratio. They are used to distinguish all the examined seven species. Conclusion ISSR-PCR Method provides a quick, reliable molecular marker technique for the identification of different species of Gynostemma Bl.

关 键 词：绞股蓝属 ISSR DNA指纹图谱 分类鉴定 

分 类 号：R282.7[医药卫生—中药学]