抗IVM多克隆抗体检测牛组织中IVM残留的研究  

Study on the Detection of Residue of Ivermectin in Bovine Tissues with Anti-ivermectin Polyclonal Antibody

作　　者：魏小娟[1] 张继瑜[1] 张梅[1] 李剑勇[1] 周绪正[1] 李金善[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所,兰州730050

出　　处：《中国奶牛》2008年第4期14-16,共3页China Dairy Cattle

基　　金：兰州牧药所科研基金(MYJJ05-3)

摘　　要：用自制的抗伊维菌素(IVM)多克隆抗体检测牛肌肉和肝脏中IVM的残留,将IVM以5.0ng/g、10.0ng/g、20.0ng/g和40.0ng/g的量分别添加至经处理的各空白组织中,用竞争ELISA法测得IVM在肌肉和肝脏中的回收率为93.9%-98.3%,变异系数为2.1%-5.1%,最低检测限为0.94ng/g。We detected the residue of Ivermectin in bovine＇s liver and muscle tissues by homemade anti-Ivermectin polyclonal antibody.We added Ivermectin to bovine tissues at levels of 5.0ng/g, 10.0ng/g,20.0ng/g and 40.0ng/g respectively and the recoveries was 93.9%～98.3% by competitive ELISA.Variation coeficient of the assay was 2.1%～5.1%,the detection limit was 0.94ng/g.

关 键 词：伊维菌素 牛组织 竞争ELISA 残留 

分 类 号：S823[农业科学—畜牧学]