寄主植物对B型烟粉虱(Bemisia tabaci)几种主要解毒酶活性的影响  

Effects of host plant on activities of some detoxification enzymes in Bemisia tabaci biotype B

作　　者：安志兰[1] 褚栋[1] 郭笃发[2] 范仲学[1] 陶云荔[1] 刘国霞[1] 张友军[3] 

机构地区：[1]山东省农业科学院高新技术研究中心,济南250100 [2]山东师范大学人口资源与环境学院,济南250014 [3]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《生态学报》2008年第4期1536-1543,共8页Acta Ecologica Sinica

基　　金：国家重点基础研究发展计划资助项目(2002CB111400);国家自然科学基金资助项目(30500331);北京市自然科学基金资助项目(6062024);山东省农业科学院创新基金资助项目(2007YCX030,2005YQ032)~~

摘　　要：研究测定了棉花、一品红、茄子和番茄4个B型烟粉虱(Bemisia tabaci)种群α-NA羧酸酯酶、β-NA羧酸酯酶、乙酰胆碱酯酶和谷胱甘肽S-转移酶活性。结果表明:α-NA羧酸酯酶活性与β-NA羧酸酯酶活性比值均大于1,说明B型烟粉虱水解α-NA的能力高于对β-NA的水解能力;B型烟粉虱不同寄主种群α-NA羧酸酯酶、β-NA羧酸酯酶活性个体分布频率均存在一定差异。B型烟粉虱番茄种群羧酸酯酶活性最高(93.06 mOD/(mg protein.min)),是棉花种群的1.49倍。B型烟粉虱茄子种群乙酰胆碱酯酶活性明显高于其他寄主种群,达极显著差异水平(p<0.01);茄子种群乙酰胆碱酯酶活性分布频率在>4U/mgprotein区间段的分布高达85%,与其他3个寄主种群分布明显不同。B型烟粉虱茄子种群谷胱甘肽S-转移酶活性最高,与其他3个寄主种群间的差异达极显著水平(p<0.01)。上述结果表明,B型烟粉虱主要解毒酶活性在不同的寄主植物上具有一定的生理可塑性。研究有利于揭示该害虫寄主范围广和寄主适应性强的生理生态学基础。The activities of α-NA carboxylesterase, β-NA carboxylesterase, acetylcholinesterase and glutathione S- transferase in laboratory populations of the whitefly Berrtisia tabaci biotype B reared on cotton, poinsettia, eggplant and tomato were examined. The ratio of α-NA carboxylesterase activity compared to β-NA carboxylesterase activity in all populations was found to be greater than 1. Further, hydrolyzation to the α-NA form was greater than the β-NA form in all 4 host populations. The frequency of c^-NA and 13-NA carboxylesterase activity in B. tabaci biotype B also differed between the four populations. The carboxylesterase activity for the tomato population was the highest at 93. 06 mOD/（ mg protein, rain）, or 1.49 times greater that for the cotton population. Acetylcholinesterase activity for the eggplant population was significantly （p 〈 （0.01） higher compared to the other 3 host populations. The frequency of acetylcholinesterase activity at 〉 4U/mg protein in the eggplant population was 85% , which was obviously different with the other 3 host populations. The glutathione S-transferase activity of the eggplant population was the highest among the 4 host populations. The difference of glutathione S-transferase activity between populations on eggplant and the other 3 was significant （p 〈 0. 01 ） at 〉 170U/rag protein. These results indicate that the detoxification enzymes in B. tabaci biotype B reared on different host plants are indicative of physiological plasticity, suggesting an ecophysiological basis for the high degree of polyphagy observed for the biotype B.

关 键 词：B型烟粉虱(Bemisia tabaci) 寄主植物 羧酸酯酶 乙酰胆碱酯酶 谷胱甘肽S转-移酶 生理可塑性 

分 类 号：S433[农业科学—农业昆虫与害虫防治]