利用原位RT-PCR技术检测库尔勒香梨中苹果茎沟病毒  

Using In Situ RT-PCR to Detect ASGV in Kuala Pear

作　　者：黄翯[1] 牛建新[1] 刘娜[1] 

机构地区：[1]石河子大学农学院园艺系,新疆石河子832003

出　　处：《石河子大学学报（自然科学版）》2008年第1期26-29,共4页Journal of Shihezi University(Natural Science)

基　　金：国家自然科学基金项目(30360066);国家科技攻关计划引导项目(2003BA546C)

摘　　要：以已知带有苹果茎沟病毒的香梨的叶片为材料,研究了香梨病毒叶片石蜡切片IS-RT-PCR检测技术。应用了直接原位RT-PCR反应,简化了试验步骤,缩短了试验周期,确定了苹果茎沟病毒在叶片中的分布位置。试验中设置了多种阴性对照,结果表现为:阴性对照组织中均未显现蓝紫色,而阳性材料组织的一些细胞表现出明显的蓝紫色,说明苹果茎沟病毒在叶片中主要分布于细胞中的栅栏组织。In the experiment, we use Kuala pear stem with apple stem grooving virus as material. And studies on detection of viral disease in Kuala pear using in situ PCR. Use direct IS RT-PCR, predigest the process and shorten the cycle. Have determined ASGV＇s distribution in leaf. In experiment many negative controls were made. The result shows：there are not blue-purple signals in negative control tissue, however, some cell expressions of the mesophyll of positive material tissues make obvious blue purple colour, which explains ASGV may distribute in palisade tissue in leaf.

关 键 词：库尔勒香梨 原位逆转录PCR 苹果茎沟病毒 

分 类 号：S436.612.19[农业科学—农业昆虫与害虫防治]