左氧氟沙星人工抗原的制备及其抗体鉴定  

Preparation of Immune Antigen and Judgement of Antibody on Levofloxacin

作　　者：蒋兴东[1] 陈玉飞[1] 涂健[1] 彭开松[1] 王涓[1] 樊磊[1] 祁克宗[1] 

机构地区：[1]安徽农业大学动物科技学院,安徽合肥230036

出　　处：《石河子大学学报（自然科学版）》2008年第1期68-71,共4页Journal of Shihezi University(Natural Science)

基　　金：安徽省"十五"科技重大攻关项目(0400303041);安徽省优秀青年基金(04041042)

摘　　要：用N-羟基琥珀酰亚胺法把左氧氟沙星与载体蛋白牛血清白蛋白偶联制备免疫抗原,用氯甲酸异丁酯法将左氧氟沙星与鸡卵清蛋白偶联,制备包被检测抗原,采用紫外扫描法对合成的抗原进行鉴定。用免疫抗原免疫大白兔获得抗左氧氟沙星多克隆抗体血清,经间接ELISA试验检测特异性抗体效价,测得效价达1:212以上。通过紫外扫描及间接ELISA试验,结果表明,制备的抗原和抗体获得成功。Levofloxacin（LVL） was coupled with carrier protein bovine serum album（BSA） to prepare the immune antigen LVL-BSA by N-hydmxysuccinimide ester（NHS） method and coupled with chicken ovalbumin（OVA） to prepare coating detection antigen by isobutyl chloroformate method. To identity its effect by using the UV scanning method. LVL-BSA as immunizing antigen immune albino rabbit to obtain polyclonal antibody for anti-levofloxacin. We use indirect ELISA experiment to detect specificity antibody titer to reach above 1 ： 2^12. The result indicated that the complete antigens were successfully obtained by using the UV scanning and indirect ELISA method.

关 键 词：左氧氟沙星 抗原 抗体 间接ELISA 

分 类 号：S859[农业科学—临床兽医学]