巨龙竹蔗糖磷酸合成酶基因(sps)的分离克隆  

Isolation and Cloning of Sucrose Phosphate Synthase Gene(sps) from Dendrocalamus sinicus

作　　者：张绍智[1] 王毅[1] 刘小烛[1] 

机构地区：[1]西南林学院资源学院,云南昆明650224

出　　处：《西南林学院学报》2008年第1期48-50,56,共4页Journal of Southwest Forestry College

基　　金：西南林学院大学生(含研究生)科技创新基金(No.200601)资助

摘　　要：根据已报道的玉米和水稻sps序列的保守区域设计并合成简并引物,以巨龙竹的cDNA第一链为模板,运用TD-PCR的方法,克隆巨龙竹的sps基因,通过测序和序列分析,得到大小为474 bp的基因片段,该片段与毛绿竹、黑麦草、六稜大麦等物种的sps基因均具有较高的同源性.The degenerating primer was designed and synthesized according to sps gene sequence of Zea mays and Oryza sativa. Taking the first cDNA strand of Dendrocalamus sinicus as template, the sps gene of this bamboo species was isolated and cloned by means of TD - PCR method, and 474 bp fragment was obtained by sequencing and cDNA sequence analysis. It was showed that the fragment was similar to the reported sps homologous genes of Bambusa oldhamii, Lolium perenne and Hordeum vulgare subspecies.

关 键 词：巨龙竹 蔗糖磷酸合成酶 简并引物 基因克隆 

分 类 号：S795.901[农业科学—林木遗传育种]