双孢菇培养基废料对绿豆生长及AM真菌侵染的影响  

Effects of Used Agaricus bisporus Culturing Medium on Plant Growth of Vigna radiate and Arbuscular Mycorrhizal Fungal Infection in Its Root System

作　　者：胡君利[1] 李博[2] 林先贵[1] 武术[1] 王俊华[1] 王一明[1] 李晶[3] 

机构地区：[1]中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室／南京土壤研究所一香港浸会大学土壤与环境联合开放实验室,江苏南京210008 [2]南京农业大学植物保护学院,江苏南京210095 [3]南京中科院跨克科技有限责任公司,江苏南京210008

出　　处：《生态与农村环境学报》2008年第2期61-65,共5页Journal of Ecology and Rural Environment

基　　金：中国科学院知识创新工程重大项目(KSCX1-YW-09-05)

摘　　要：通过盆栽试验，研究添加不同比例双孢菇（Agaricus bisporus）培养基废料（菇渣）对绿豆（Vigna radiate）生长及其根系受AM真菌侵染的影响。结果发现，在接种AM菌剂条件下，添加菇渣在基质中所占质量分数为5％～50％时，不同程度地提高了AM真菌对绿豆根系的侵染率，促进了植株生长与绿豆经济产量的提高，AM真菌侵染率在35d左右达到高峰。添加20％菇渣的处理效果最佳，60d收获时比对照植株高58％，生物量增加128％，豆荚干重增加598％，绿豆干重增加577％，经济产量（绿豆干重）与35d时AM真菌对绿豆根系的侵染率呈显著线性回归关系（P〈0．05）。这说明添加20％左右的菇渣最有利于促进AM真菌对绿豆根系的侵染以及绿豆植株的营养生长和生殖生长。在最适菇渣比例（20％）条件下接种AM菌剂，基质灭菌（包括土壤与菇渣）处理的AM真菌侵染率与非灭菌对照没有显著差异，表明双孢菇培养基废料可以应用于AM菌剂的扩繁。A pot culture experiment was carried out to study effects of application rate of used Agaricus bisporus culturing medium, called mushroom castoff （MC） , on plant growth of Vigna radiate and arbuscular mycorrhizal fungal infection in its root system. With arbuscular mycorrhizal fungi（AMF） inoculation, the addition of MC at a rate of 5% to 50% increased AMF infection rate, plant biomass and economic yield to a varying extent. The infection rate peaked around D35 after sowing. The addition of MC at a rate of 20% benefited the plant in growth the most, compared to the controls, the shoot length increased by 58% , plant biomass increased by 128% , bean pod dry weight increased by 598% and bean dry weight increased by 577% on D60 when the plant was harvested. A significant linear regression relationship was observed between the economic yield （ bean dry weight） and the AMF infection rate on D35 （ P 〈 0. 05 ）, which suggests that application of MC at a rate of ca. 20% benefits the most vegetative and productive growth of the plant and AMF infection in its root system. Besides, no significant difference was found between sterilized and non-sterilized substrates in AMF infection rate in Vigna radiate root system, which indicates that MC accelerates AM infection in plant roots and, therefore, propagation of AMF.

关 键 词：菇渣 双孢菇培养基废料 绿豆 经济产量 AM真菌侵染率 AM菌剂扩繁 

分 类 号：X712[环境科学与工程—环境工程] X705