耐甲氧西林金黄色葡萄球菌多重耐药研究  

Multi-antibiotic resistance of Methicillin-resistant Staphylococcus aureus

作　　者：宋焰桃[1] 徐元宏[1] 

机构地区：[1]安徽医科大学第一附属医院检验科

出　　处：《中南药学》2008年第2期246-248,共3页Central South Pharmacy

基　　金：安徽省教育厅自然基金重点课题资助(KJ2007A030)

摘　　要：目的了解本地区耐甲氧西林金黄色葡萄球菌(MRSA)的多重耐药情况。方法收集经头孢西丁纸片扩散试验和青霉素结合蛋白2a胶乳凝集实验分离出的51株MRSA,用β-内酰胺酶实验、D试验、Kirby-Bauer法检测MRSA产β-内酰胺酶以及对红霉素、克林霉素、4种氟喹诺酮类药物(氧氟沙星、诺氟沙星、左旋氧氟沙星、加替沙星)和万古霉素耐药性。结果51株MRSA头孢西丁纸片抑菌圈直径介于6～20mm,产β-内酰胺酶率为84.3%,对红霉素、克林霉素、氟喹诺酮类、万古霉素耐药率分别为98.0%、86.3%、88.2%、0,红霉素诱导克林霉素耐药率为50.0%(3/6)。结论MRSA呈多重耐药,轻度感染可根据药敏结果选万古霉素与氟喹诺酮类?生素联合用药。Objective To study the situation of Methicillin-resistant Staphylococcus aureus （MRSA） resistant to multiantibiotics in the local area. Methods Fifty-one strains of MRSA detected by cefoxitin disk diffusion and penicillin binding protein 2a latex agglutination test were determined with β-lactamase test, D-test, Kirby-Bauer methods with erythromycin （ERY）, clindamycin （DA）, 4 fluoroquinolones （Ofloxacin （OFX）, Norfloxacin （NOR）, Levofloxacin （LEV）, Gatifloxacin （GTX））, and Vancomycin （VAN）. Results The inhibitor diameters of cefoxitin disk of 51 strains of MRSA were 6-20 mm. β-lactamase producing rate and the resistance rate to ERY, DA, Fluoroquinolones, and VAN were 84.3%, 98.0%, 86.3X, 88.2%, and 0, respectively. The ratio of erythromycin inducible clindamycin resistance was 50. 0% （3/6）. Conclusion MRSA are resistant to multiple antimicrobial agents. Vancomycin associating with fluoroquinolones antibiotics could been adopted in low level MRSA infections according to antimicrobial susceptibility testing.

关 键 词：耐甲氧西林金黄色葡萄球菌 头孢西丁纸片扩散 PBP2a胶乳凝集 β-内酰胺酶 红霉素诱导克林霉素耐药(D试验) 氟喹诺酮类抗生素 万古霉素 多重抗药性 

分 类 号：R37[医药卫生—病原生物学]