细胞间黏附分子-1在急性肝内胆汁淤积性肝损伤大鼠模型中的表达  

Expression of Intercellular Adhesion Molecule-1 in Rat Model with Acute Intrahepatic Cholestatic Hepatic Injury

作　　者：丁艳[1] 黄志华[1] 赵雷[2] 梅红[3] 

机构地区：[1]华中科技大学同济医学院附属同济医院儿科,武汉430030 [2]华中科技大学同济医学院附属协和医院感染科,武汉430022 [3]武汉市儿童医院消化科,武汉430016

出　　处：《实用儿科临床杂志》2008年第7期504-506,512,共4页Journal of Applied Clinical Pediatrics

基　　金：湖北省自然科学基金项目资助(2007ABA080)

摘　　要：目的探讨细胞间黏附分子-1(ICAM-1)在急性肝内胆汁淤积性肝损伤大鼠模型中的表达及其意义。方法雄性SD大鼠α-异硫氰酸萘酯(ANIT)50 mg/kg一次灌胃,建立急性肝内胆汁淤积性肝损伤动物模型,实时荧光定量PCR检测灌胃后大鼠24、48、72 h肝组织中ICAM-1 mRNA表达量,全自动生化分析仪检测其血清总胆红素(TB)、结合胆红素(DB)、ALT、γ-谷氨酰转肽酶(GGT)水平。取大鼠肝组织行病理学检查。采用SPSS12.0软件进行统计学分析。结果模型组24、48、72 h肝组织ICAM-1 mRNA表达量分别为3.43±0.58、5.32±0.67、2.54±0.41,均显著高于对照组(Pa<0.05);模型组24、48 h肝组织ICAM-1 mRNA表达量分别与同一时间点的血清TB、DB、ALT、GGT水平呈正相关(Pa<0.05),模型组72 h肝组织ICAM-1mRNA表达量与其72 h时血清TB、DB、ALT、GGT水平无相关性(Pa>0.05);模型组24、48、72 h肝组织ICAM-1 mRNA表达量均与同一时间点肝组织病理损伤程度分级呈正相关(Pa<0.05)。结论ICAM-1在肝内胆汁淤积性肝损伤大鼠肝组织中基因表达增高,其参与了胆汁淤积性肝损伤的病理过程。Objective To explore the expression of intercellular adhesion molecule - 1 （ ICAM - 1 ） in rat model with acute intrahepatic cholestatic hepatic injury and its significance. Methods A single dose （50 mg/kg） of α- naphthylisothiocyanate（ANIT） was administered by gavage to each experimental rat to induce intrahepatic cholestasis. The specimens of hepatic tissue and serum were collected from rats at 24,48, and 72 h after intoxication. The value of ICAM - 1 mRNA and serum total bilirubin （ TB ）, direct bilirubin （ DB ）, alanine aminotransferase （ ALT）, glutamyltranspeptidase （GGT） were assayed by real - time PCR and fully - auto chemistry analyzer, respectively. Hepatic tissue were examined by pathologic method. SPSS 12.0 software was used to analyze the data. Results Of model group at 24,48,72 h after intoxication,the values of ICAM - 1 mRNA were 3.43 ± 0.58,5.32 ± 0.67,2.54 ± 0.41, respectively,which were higher than those of control group （P 〈 0.05 ）. In model group at 24,48 h after intoxication, the value of ICAM - 1 mRNA was positively correlated to that of serum TB, DB, ALT, GGT （ P 〈 0.05 ） ; In model group at 72 h after intoxication, the value of ICAM - 1 mRNA had no correlation to that of serum TB, DB, ALT, GGT （ P 〉 0.05 ） ; In model group at 24,48,72 h after intoxication, the value of ICAM - 1 mRNA was positively correlated to the degree of pathological injury in hepatic tissue （ Pa 〈 0.05 ）. Conclusions Acute intrahepatic cholestatic hepatic injury induced by ANIT results in alterations of ICAM - 1 gene expression in hepatic tissue. ICAM - 1 is involved in pathological progress of intrahepatic cholestatic hepatic injury.

关 键 词：细胞间黏附分子-1 基因 胆汁淤积 实时荧光定量反转录聚合酶链反应 

分 类 号：R725.7[医药卫生—儿科]