维生素A与儿童腹泻的关系  

Relationship between Vitamin A and Diarrhea in Children

作　　者：孙吉萍[1] 赵慧欣[1] 谈藏文[1] 吴光驰[1] 

机构地区：[1]首都儿科研究所附属儿童医院内科,北京100020

出　　处：《实用儿科临床杂志》2008年第7期532-533,557,共3页Journal of Applied Clinical Pediatrics

摘　　要：目的探讨血清维生素A（Vit A）水平与腹泻病的关系。方法血清Vit A水平测定方法是在暗光条件下采集患儿入院次日、入院第7天及健康儿童晨起空腹指血,以3000 r/min分离血清,置-20℃冰箱避光冻存。用美国Sigma公司生产的Vit A标准血清,日本岛津RF-540荧光分光光度计,在激发光波长330 nm,发射光波长480 nm处测定。平均回收率102.4%,变异系数2.0%。分析本院收治的205例腹泻患儿血清Vit A水平变化及相关因素,选择同期保健科体检的健康儿童162名作为对照。亚临床Vit A缺乏（SVAD）诊断标准：血清Vit A 0.35～0.70μmol/L。结果腹泻患儿血清Vit A水平[（0.70±0.40）μmol/L]明显低于健康儿童[（1.90±0.60）μmol/L]（P〈0.01）。SVAD检出率为54.10%,明显高于健康对照组1.0%（P〈0.05）。腹泻儿童血清Vit A水平在入院第7天较入院时明显升高,腹泻患儿的血清Vit A在平均体温≥39℃,有脱水,入院次日大便10次/d组为低。SVAD检出率以体温≥39℃、有脱水组为高,与发热、脱水、腹泻次数相关。短时期Vit A干预治疗对腹泻病程无影响,但与近3个月内患儿感染性疾病的发病率相关。结论Vit A缺乏易导致腹泻病的发生,而腹泻会进一步降低患儿原本不高的血清Vit A水平,建议腹泻患儿应补充Vit A。Objective To explore the relationship between the level of serum Vit A （ Vit A） and diarrhea in children. Methods The determination of the serum Vit A contents：at the scotopia condition, peripheral blood was taken from diarrhea children（ that in the first hospital - day and in the seventh hospital - day） and healthy children, and was separated by the centrifuge at 3 000 r/min, and was be stored - 20 ℃ refrigerator and avoid light. Level variance of serum Vit A and the correlation factors were analyzed by RF - 540 spectrophotofluorometer （ SHI- MADZU. Japan） ,the Vit A standard serum （Sigma. USA） as reference. At exciting light wavelength standards 330 nm, transmit light wavelength standards 480 nm, the serum Vit A was determined. Mean recovery ratio was 102.4% , coefficient of variability was 2.0%. The healthy children from the department of health were chosen as control group. Diagnosis standard of subclinical Vit A deficiency（SVAD） was the serum Vit A 0.35 - 0.70 μmol/L. Results The level of Vit A of diarrhea children [ （ 0.70 ± 0.40 ） μmol/L ] was obviously lower than that in healthy children [ （ 1.90 ± 0.60 ） μmol/L ] （ P 〈 0.01 ）. SVAD （ 54.10% ） was obviously higher than that in healthy children （ 1.0% ） （ P 〈 0.05 ）. The level of Vit A of diarrhea children was obviously higher than that in hospital after the seventh day, and also the Vit A level was lower in the patients of high fever, dehydration and high -frequency diarrhea. The level of Vit A of diarrhea children was lower with temperature ≥39 ℃ and dehydration and stool ≥ 10 times/day. The SVAD was more often found in the high -fever（ temperature ≥39 ℃ and dehydration patients in diarrhea. Short period of intervention treatment didn＇t influence the course of diseases, but it was correlated with the incidence rate of infectious diseases within 3 months. Conclusions The deficiency of Vit A may result in diarrhea, diarrhea can diminish the level of Vit A,the diarrhea children should be

关 键 词：维生素A 腹泻 高热 脱水 大便次数 

分 类 号：R725.7[医药卫生—儿科]