茶叶的冷驯化反应及其与Ca^(2+)的关系  

The Responses of Tea to Cool Acclimation and its Association with Ca^(2+)

作　　者：李先文[1] 袁正仿[1] 

机构地区：[1]信阳师范学院生命科学学院,河南信阳464000

出　　处：《信阳师范学院学报（自然科学版）》2008年第2期213-216,共4页Journal of Xinyang Normal University(Natural Science Edition)

基　　金：河南省科技攻关项目(0224050031)

摘　　要：以低温半致死温度(LT50)和膜脂过氧化程度为指标,检测了薮北茶(Yabu Kita)的冷驯化反应,并结合CaC l2、Ca2+螯合剂EGTA、CaM拮抗剂氯丙嗪(CPZ)的处理,探索了薮北茶的低温反应与Ca2+之间的关系.结果发现:(1)薮北茶对低温的感受较为灵敏,在冷驯化4 h后,LT50开始降低,并随着冷驯化时间的延长(4~72h),LT50呈现不断降低的趋势;(2)薮北茶对低温的反应与Ca2+和CaM等Ca2+激活蛋白类有关;(3)茶叶对CPZ的反应很敏感.The reaction of Yabu Kita to cool acclimation was investigated with half lethal low temperature（ LT50 ） and lipid over oxidation as the criterion. The relationship of low temperature responses of Yabu K/ta and Ca^2＋ was studied with the treatment of CaC12 .EGTA （chelator of Ca^2＋ ）or CPZ （antagonist of Ca^2＋ activation protein）. The results show： （ 1 ） YabuKita is sensitive to low temperature. Its LT50 begins to decrease after 4 h in cool domestication and drops gradually along with cold domestication （4 -72 h ）. （2） Ca^2＋. CaM and Ca^2＋ -activated protein kinases have effects on the responses of Yabu Kita getting to low temperature. （3）Tea is sensitive to CPZ .

关 键 词：茶叶 冷驯化 低温半致死温度 膜脂的过氧化 

分 类 号：Q945.78[生物学—植物学]