几种麻类作物及其近缘种植物总DNA的提取与鉴定  

Isolation and Characterization of Total DNA from Several Fibre Crops and Their Allies

作　　者：郭安平[1] 黄明[2] 郑学勤[3] 彭世清[3] 程新奇[1] 黎宇[1] 

机构地区：[1]中国农业科学院麻类研究所,湖南沅江413100 [2]广西师范大学生物系,广西桂林54104 [3]中国热带农业科学院热带作物生物技术国家重点实验室,海南儋州571737

出　　处：《中国麻作》1997年第4期4-10,共7页China's Fiber Crops

摘　　要：采用Pich和 Schubert 1993年发展的SDS方法，经适当改进后，成功地从苎麻、红麻、黄麻、青麻四种麻类作物及其近缘种植物中提取了细胞总DNA，并对其得率、质量和纯度进行了鉴定。所得的DNA可进行限制性内切酶消化、PCR扩增和RAPD分子标记。本研究发展的这一快速、简便、适于麻类作物及其近缘种植物的总DNA提取方法，将有助于在DNA分子水平研究麻类种质资源的遗传多样性。<Abstrsct>A DNA extraction procedure namely SDS which had been developed by Pich and Schubery in 1993was improved and successfully used to obtain total DNA from Boehmeria. nivea (L ) Gasud, Hibiscus.cannabinus, Corchorus. olitorius, Corchorus. capsularis, Abutilon avicennae Gaertner and their -closespecies plants. The DNA yield, quality and purity were characterized, these isolated DNA could be digested with restiction enzymes and used for PCR amplification or RAPD analysis which are useful asmolecular genetyc makers. A fast, inexpensive procedure for extraction of high quality DNA from fibrecrops and their allies has been developed in this study,which is helpful for detecting the genetic diversityof those kind of plants in the field of DNA molecules.

关 键 词：麻类作物 DNA提取 限制性内功酶 PCR 

分 类 号：S563[农业科学—作物学]