青花菜高频离体再生体系的研究  

The high frequency of shoot regeneration in Brassica oleracea L.var.italica in vitro

作　　者：张丽丽[1] 张蜀宁[1] 吴震[1] 汤伟华[1] 杨学东[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,江苏南京210095

出　　处：《南京农业大学学报》2008年第2期41-44,共4页Journal of Nanjing Agricultural University

基　　金：国家863计划资助项目(2003AA207120)

摘　　要：以青花菜04J-20、04J-19和04J-21带柄子叶为外植体，研究其高频离体再生体系。比较了不同基因型、不同质量浓度植物生长调节剂、不同时期苗龄外植体对不定芽再生频率的影响。结果表明：04J-20 8d苗龄外植体不定芽的再生频率最高为100％（MS＋0．10mg·L^-1NAA＋6．0mg·L^-1 6BA＋8g·L^-1琼脂＋30g·L^-1蔗糖），继代培养增殖系数达8～9（MS＋0．2mg·L^-1NAA＋2．0mg·L^-1 6-BA＋7g·L^-1琼脂＋30g·L^-1蔗糖），生根培养诱导生根率为100％（1／2MS＋0．1mg·L^-1NAA＋7g·L^-1琼脂＋30g·L^-1蔗糖），驯化移栽成活率达95％，获得增殖200倍的田间再生植株。The high frequency of shoot regeneration in broccoli of cotyledons with petiole as explants was studied. The result showed that the material of 04J-20 was the highest frequency of 100% to the cotyledons with petiole, when the explants were precultured for 8 days and then placed on the MS medium supplemented with 6. 0 mg · L^-1 6-BA, 0. 10 mg · L^-1 NAA, 8 g · L^-1 agar and 30 g · L^-1 sucrose. Frequent subcultures of existing shoots and shoot clusters to medium containing 0. 2 mg · L^-1 NAA and 2. 0 mg · L^-1 6-BA promoted rapid shoot multiplication （ proliferating about eight to nine times）. The use of 0. 1 mg · L^-1 NAA in the rooting medium increased the number of healthy roots per rooted plant （ root ratio was 100% ）. Rooted plantlets were transferred to soil-less mixture in pots. Applying this protocol, rooted plantlets proliferating 200 times were obtained. It was also studied on the effects of different concentration of plant hormones, seedling ages and genotypes on shoot regeneration.

关 键 词：青花菜 带柄子叶 离体培养 高频再生 

分 类 号：S634.3[农业科学—蔬菜学]