无指盘臭蛙皮肤分泌液中促胰岛素释放肽的分离纯化与活性初步研究  

Isolation and purification of a novel insulinotropic peptide from the skin secretions of Odorrana grahami and the study of its bioactivities

作　　者：李宗杰[1] 李建许[2] 王梅玉[3] 吕毅 张崇星[1] 张克云[1] 洪晓月[3] 赖仞[1] 

机构地区：[1]南京农业大学生命科学学院,江苏南京210095 [2]中国科学院昆明动物研究所,云南昆明650223 [3]南京农业大学植物保护学院,江苏南京210095

出　　处：《南京农业大学学报》2008年第2期144-148,共5页Journal of Nanjing Agricultural University

基　　金：江苏省自然科学基金(BK2005422);云南省自然科学基金(2006C0011Z)

摘　　要：通过葡聚糖Sephadex G-50凝胶过滤和反相高压液相层析等手段,对无指盘臭蛙皮肤分泌液进行分离纯化,得到了高纯度的多肽分离峰。分别采用体外培养的大鼠胰岛和INS-1胰岛β细胞系检测分离峰的促胰岛素释放活性。结果表明,所得多肽既可促进原代培养的大鼠胰岛分泌胰岛素,又可以促进INS-1细胞胰岛素释放;运用飞行质谱仪测定该促胰岛素释放肽的相对分子质量,其大小为1 868。此外,利用鸡胚尿囊绒毛膜(CAM)体内模型检测,发现该多肽还具有显著的抑制血管生成活性。Insulinotropic peptides were isolated and purified from the skin secretions Odorrana grahami by gel filtration and RP-HPLC, and many purified peaks were obtained. Rat pancreatic islets and rat insulinoma INS-1 cells were used to assay the insulinreleasing activity of the isolated peaks, and one peptide was found to have significant insulin-releasing activity on both rat pancreatic islets and rat insulinoma INS-1 cells. Then the molecular weight was determined by fast atom bombardment （FAB） mass spectrometry to be 1 868. Furthermore, the chick chorioallantoic membrane （CAM） assay was carried out to verify angiogenesis inhibiting activity of the peptide, and it showed obvious angiogenesis inhibiting activity by preventing the growth of newborn small vessels.

关 键 词：无指盘臭蛙 促胰岛素释放肽 胰岛素分泌 糖尿病 

分 类 号：R331[医药卫生—人体生理学]