表达HPV16 L1、L2和E7蛋白的非复制重组痘苗病毒的构建  

Construction of Non-replicating Recombinant Vaccinia Virus Expressing HPV16 L1,L2E7 Proteins

作　　者：范江涛[1] 陈心秋[2] 黄薇[2] 田厚文[3] 

机构地区：[1]广西医科大学第一附属医院妇科,南宁530021 [2]广西医科大学附属肿瘤医院妇瘤科 [3]中国疾病预防控制中心病毒病研究所

出　　处：《肿瘤防治研究》2008年第4期229-232,共4页Cancer Research on Prevention and Treatment

基　　金：国家高技术研究发展计划863基金资助项目(2002AA216041)

摘　　要：目的构建防治宫颈癌的表达人乳头瘤病毒16型(HPV16)L1,L2E7蛋白的非复制重组痘苗病毒疫苗株。方法以痘苗病毒为载体,利用同源重组技术筛选共表达HPV16L1,L2E7蛋白的重组痘苗病毒,用PCR和Western blot技术进行鉴定。结果该病毒在鸡胚成纤维细胞(CEF)中连续传代15代,经PCR检测证实病毒基因组中有HPV16L1,L2E7目的基因插入,Western blot检测证实病毒可以稳定表达L1和L2E7蛋白。结论构建的病毒NTVJL1L2E7可以在真核细胞中表达HPV16L1和L2E7蛋白,可以作为治疗和预防HPV16相关疾病的候选疫苗。Objective To construct a non-replicating vaccinia virus expressing HPV16 L1, L2E7 proteins as a candidate vaccine for cervical cancer. Methods Using vaccinia virus vector, we generated a strain of non-replicating recombinant vaccinia virus vaccine expressing HPV16 L1, L2E7 proteins by homologous recombination and identified by PCR and Western blot. Results We demonstrated that the L1, L2E7 gene of HPV16 were integrated into vaccinia genosome and could express L1, L2E7 protein stably when infected the CEF using PCR and Western blot assay. Conclusion NTVJL1/L2E7 can express L1, L2E7 protein of HPV16 and can be taken as a candidate vaccine for HPV16 associated diseases.

关 键 词：人乳头瘤病毒 痘苗病毒 疫苗 

分 类 号：Q753[生物学—分子生物学]