加热毛细管作为飞行时间质谱仪真空接口的研究  

Study of a Capillary Interface for a Home-made Time-of-flight Mass Spectrometer

作　　者：郭长娟[1] 黄正旭[1] 高伟[1] 粘慧青[1] 周振[1] 

机构地区：[1]中国科学院广州地球化学研究所有机地球化学国家重点实验室

出　　处：《分析测试学报》2008年第4期339-343,共5页Journal of Instrumental Analysis

基　　金：广东省科技攻关计划资助项目(2003B12007);广州市科技攻关计划资助项目(2004Z2-D9051)

摘　　要：介绍了一种自制的毛细管真空接口,用于连接电喷雾离子源(electrospray ionization,ESI)与自制高分辨率垂直引入式飞行时间质谱仪,初步研究了该接口对仪器灵敏度的影响。结果表明,毛细管接口在有效提高离子传输效率的同时也提高了质谱仪真空系统的性能。加热毛细管的最优加热温度为125℃,仪器最低检测灵敏度为1.6 amol。该文还介绍了该接口与自制大气压基体辅助激光解析离子源(atmospheric pressure ma-trix-assisted laser desorption/ionization,AP-MALDI5)联用的实验结果,仪器与该离子源联用的最低检测灵敏度为25 fmol。A home-made capillary interface has been coupled with a high resolution orthogonal-injection time-of-flight mass spectrometer. The performance of the interface on the intensity of spectra was evaluated with electrospray ionization. It has been shown that the capillary interface can not only increase the ion translation efficiency, but also improve the vacuum condition of the mass spectrometer. The optimized temperature of the heated capillary was 125 ℃, and the detection limit of the instrument is 1. 6 amol. Coupling atmospheric pressure matrix-assisted laser desorption ionization with the improved interface resulted in an instrument detection limit as low as 25 fmol.

关 键 词：飞行时间质谱仪 加热毛细管接口 灵敏度 自制 

分 类 号：O657.63[理学—分析化学]