稻瘟病菌的菌丝融合现象及其后代的致病性变异  

The Phenomenon of Hyphal Fusion in Magnaporthe grisea and the Pathogenicity of Its Progeny

作　　者：沈瑛[1,2] 李成云 袁筱萍 赵新华[1,2] 罗朝喜[1,2] 王艳丽 

机构地区：[1]中国水稻研究所 [2]云南省农业科学院

出　　处：《中国农业科学》1997年第6期16-22,共7页Scientia Agricultura Sinica

基　　金：国家攀登计划项目;云南省科委应用基础研究重点基金

摘　　要：用重复序列探针ＭＧＲ５８６与限制性内切酶ＥｃｏＲ１组合，分析了１１个稻瘟病菌（无性／有性）菌丝自身融合的亲代菌株及其２２个菌丝融合点的再度培养菌株，以及７对相互组配后的第５代菌丝融合菌株的限制性片段长度多态性（ＲＦＬＰｓ），依其ＭＧＲ－ＤＮＡ指纹的相似性，其中大多数菌株被归属于不同的系谱；结合病菌的致病性测定，亦可鉴别出许多互不相同的生理小种。初步证实了稻瘟病菌本身存在的菌丝融合现象是该菌生理小种致病性变异的原因之一。The MGRDNA fingerprintings of 40 isolates in Magnaporthe grisea have been studied with a conversed repetitive sequence probe MGR586 /EcoR1 digest combination, including 11 tested parental isolates (asexual / sexual) with hyphal fusion ability, 22 recultural isolates of hyphal fusion spots and the 5th generational isolates of 7 paired isolates cross together. Among them, most of the isolates with hyphal fusion have been distinguished into several different lineages based on MGRDNA fingerprint similarity. Compared with the results of testing pathotypes, the different pathotypes could be identified as well. The preliminary results obtained in the study indicated that the phenomenon of hyphal fusion presented in P. grisea is one of the reasons for the pathogenic variation. 

关 键 词：水稻 稻瘟病菌 致病型 菌丝融合 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]