阿霉素纳米粒对MRP介导的膀胱肿瘤多药耐药细胞株EJ/MRP多药耐药性的逆转作用  

Reversal Effect of Adriamycin-loaded Nanoparticles on MRP-mediated Bladder Tumor Multidrug Resistance in Cell Line EJ/MRP

作　　者：王磊[1] 柯红[2] 崔洁[2] 

机构地区：[1]三峡大学医学院,湖北宜昌443002 [2]三峡大学第三临床医学院.葛洲坝中心医院,湖北宜昌443002

出　　处：《中国药业》2008年第9期14-16,共3页China Pharmaceuticals

摘　　要：目的研究阿霉素纳米粒对多药耐药相关蛋白(MRP)介导的膀胱肿瘤多药耐药的逆转作用。方法采用四甲基偶氮唑盐(MTT)法测定药物的体外杀伤作用,应用流式细胞术测定细胞内药物浓度。结果阿霉素纳米粒对EJ细胞的细胞毒作用与阿霉素相似,EJ/MRP细胞对阿霉素纳米粒较阿霉素敏感4.00倍。结论阿霉素纳米粒通过增加耐药细胞内阿霉素浓度而有效逆转多药耐药。Objective To investigate the reversal effect of adriamycin- loaded nanoparticles on MRP- mediated bladder tumor multidrug resistance （MDR）. Methods The reversal effect of the drugs on multidrug-resistance was determined by MTT method. Intracellular accumulation of ADR was studied by flow cytometry. Results The cytotoxicity of adriamycin- loaded nanoparticles to EJ cells was compatible with adriamycin,but the chemosensitivity of EJ/MRP cells to adriamycin-loaded nanoparticles was increased to 4. 00 times compared with that of EJ/MRP cells to free adriamycin. Conclusion Adriamycin- loaded nanoparticles reverse MDR effectively by increasing intracellular accumulation of adriamycin in EJ/MRP cells.

关 键 词：阿霉素 聚氰基丙烯酸异丁酯 纳米粒 多药耐药 逆转 多药耐药相关蛋白 

分 类 号：R944.9[医药卫生—药剂学] R965[医药卫生—药学]