Development and Characterization of Microsatellite Markers for the Pacific Abalone (Haliotis discus) via EST Database Mining  

作　　者：ZHAN Aibin BAO Zhenmin WANG Mingling CHANG Dan YUAN Jian WANG Xiaolong HU Xiaoli LIANG Chengzhu HU Jingjie 

机构地区：[1]Laboratory of Marine Genetics and Breeding （MGB）, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China [2]Shangdong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266002, P R. China

出　　处：《Journal of Ocean University of China》2008年第2期219-222,共4页中国海洋大学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.30500379);Grant of the Inspection Technologies for Human and Animal Resources(2005IK053-3);the Student Research Training Program(0611010401)

摘　　要：The EST database of the Pacific abalone (Haliotis discus) was mined for developing microsatellite markers. A total of 1476 EST sequences were registered in GenBank when data mining was performed. Fifty sequences (approximately 3.4%) were found to contain one or more microsatellites. Based on the length and GC content of the flanking regions, cluster analysis and BLASTN, 13 microsatellite-containing ESTs were selected for PCR primer design. The results showed that 10 out of 13 primer pairs could amplify scorable PCR products and showed polymorphism. The number of alleles ranged from 2 to 13 and the values of Ho and He varied from 0.1222 to 0.8611 and 0.2449 to 0.9311, respectively. No significant linkage disequilibrium (LD) between any pairs of these loci was found, and 6 of 10 loci conformed to the Hardy-Weinberg equilibrium (HWE). These EST-SSRs are therefore potential tools for studies of intraspecies variation and hybrid identification.The EST database of the Pacific abalone （Haliotis discus） was mined for developing microsatellite markers. A total of 1476 EST sequences were registered in GenBank when data mining was performed. Fifty sequences （approximately 3.4%） were found to contain one or more microsatellites. Based on the length and GC content of the flanking regions, cluster analysis and BLASTN, 13 microsatellite-containing ESTs were selected for PCR primer design. The results showed that 10 out of 13 primer pairs could amplify scorable PCR products and showed polymorphism. The number of alleles ranged from 2 to 13 and the values of Ho and He varied from 0.1222 to 0.8611 and 0.2449 to 0.9311, respectively. No significant linkage disequilibrium （LD） between any pairs of these loci was found, and 6 of 10 loci conformed to the Hardy-Weinberg equilibrium （HWE）. These EST-SSRs are therefore potential tools for studies of intraspecies variation and hybrid identification.

关 键 词：MICROSATELLITE Pacific abalone Haliotis discus EST 

分 类 号：Q959.4[生物学—动物学]