苦参药材高效液相指纹图谱分析方法研究  

Studies on Analyzing of HPLC Fingerprint for Sophora Flavescens Ait

作　　者：徐今宁[1] 蒋贵昱[1] 汤建华[1] 

机构地区：[1]河北北方学院附属第一医院药剂处,河北张家口075000

出　　处：《河北北方学院学报（医学版）》2008年第2期15-18,共4页Journal of Hebei North University:Medical Edition

基　　金：河北省医学科学研究重点课题计划(编号:06110)

摘　　要：目的:探讨一种评价苦参质量的高效液相指纹图谱分析方法的可行性及其应用价值。方法:采用高效液相色谱方法,Hypersil C18(250mm×4.6mm,5μm)色谱柱,以甲醇-乙腈-20mmol/L磷酸二氢钾溶液(加三乙胺调pH至8.0)为流动相,梯度洗脱。流速为0.8 mL.min-1,柱温:30℃;检测波长220nm,建立苦参药材HPLC指纹图谱共有模式,并对不同产地药材进行了相似度比较。结果:建立了苦参药材HPLC指纹图谱共有模式,并对不同产地药材进行相似度比较。结论:本研究建立的指纹图谱检测方法简便、快捷、可靠,可用于苦参药材的质量评价。Objective：To establish a method for analyzing HPLC fingerprints of Sophora flavescens Ait. Methods：The HPLC method was used with Hypersil C18 column （250mm× 4. 6mm,5μm）. The mobile phases were consisted of methanob acetonitrile -20mmol/L KH2PO4 solution （adjusted pH to 8. 0 with triethylamine） respectively,gradient elute. The flow rate was 0. 8mL· min^-2. The temperature of column was 30℃. The UV detection wavelength was 220nm. Results：The mutual mode of HPLC fingerprints was set up and the similar degrees to the crude drugs of different habitats were compared. Conclusion： The method was simple andreliable,and it was capable of effectively controlling the quality of Sophora flavescens Ait.

关 键 词：苦参 色谱法/液相 图谱 

分 类 号：R9[医药卫生—药学]